Publication detail

Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T

SEDLÁŘ, K. NYKRÝNOVÁ, M. BEZDÍČEK, M. BRANSKÁ, B. LENGEROVÁ, M. PATÁKOVÁ, P. ŠKUTKOVÁ, H.

Original Title

Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T

Type

journal article in Web of Science

Language

English

Original Abstract

Clostridium beijerinckii is a relatively widely studied, yet non-model, bacterium. While 246 genome assemblies of its various strains are available currently, the diversity of the whole species has not been studied, and it has only been analyzed in part for a missing genome of the type strain. Here, we sequenced and assembled the complete genome of the type strain Clostridium beijerinckii DSM 791T, composed of a circular chromosome and a circular megaplasmid, and used it for a comparison with other genomes to evaluate diversity and capture the evolution of the whole species. We found that strains WB53 and HUN142 were misidentified and did not belong to the Clostridium beijerinckii species. Additionally, we filtered possibly misassembled genomes, and we used the remaining 237 high-quality genomes to define the pangenome of the whole species. By its functional annotation, we showed that the core genome contains genes responsible for basic metabolism, while the accessory genome has genes affecting final phenotype that may vary among different strains. We used the core genome to reconstruct the phylogeny of the species and showed its great diversity, which complicates the identification of particular strains, yet hides possibilities to reveal hitherto unreported phenotypic features and processes utilizable in biotechnology.

Keywords

butanol; ABE; IBE; core genome; accessory genome; pan genome

Authors

SEDLÁŘ, K.; NYKRÝNOVÁ, M.; BEZDÍČEK, M.; BRANSKÁ, B.; LENGEROVÁ, M.; PATÁKOVÁ, P.; ŠKUTKOVÁ, H.

Released

10. 7. 2021

Publisher

MDPI

ISBN

2227-9717

Periodical

Processes

Year of study

9

Number

7

State

Swiss Confederation

Pages from

1

Pages to

16

Pages count

16

URL

https://www.mdpi.com/2227-9717/9/7/1196

Full text in the Digital Library

http://hdl.handle.net/11012/200480

BibTex

@article{BUT171987,
  author="Karel {Sedlář} and Markéta {Nykrýnová} and Matěj {Bezdíček} and Barbora {Branská} and Martina {Lengerová} and Petra {Patáková} and Helena {Vítková}",
  title="Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T",
  journal="Processes",
  year="2021",
  volume="9",
  number="7",
  pages="1--16",
  doi="10.3390/pr9071196",
  issn="2227-9717",
  url="https://www.mdpi.com/2227-9717/9/7/1196"
}

Documents

processes-09-01196.pdf