Publication result detail

Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil

POSPÍŠILOVÁ, A.; NOVÁČKOVÁ, I.; PŘIKRYL, R.

Original Title

Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil

English Title

Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil

Type

WoS Article

Original Abstract

The aim of this work was to develop a soap-based method for the isolation of poly(3-hydroxybutyrate) from bacterial biomass. The method consisted of adding soap derived from waste cooking oil to a concentrated (25%) biomass suspension, heating and centrifugal separation. Purity above 95% could be achieved with soap:cell dry mass ratios at least 0.125 g/g, making the method comparable to other surfactant-based protocols. Molecular weights M, of products from all experiments were between 350 and 450 kDa, being high enough for future material applications. Addition of hydrochloric acid to the wastewater led to the precipitation of soap and part of non-P3HB cell mass. The resulting precipitate was utilized as a carbon source in biomass production and increased substrate-to-P3HB conversion.

English abstract

The aim of this work was to develop a soap-based method for the isolation of poly(3-hydroxybutyrate) from bacterial biomass. The method consisted of adding soap derived from waste cooking oil to a concentrated (25%) biomass suspension, heating and centrifugal separation. Purity above 95% could be achieved with soap:cell dry mass ratios at least 0.125 g/g, making the method comparable to other surfactant-based protocols. Molecular weights M, of products from all experiments were between 350 and 450 kDa, being high enough for future material applications. Addition of hydrochloric acid to the wastewater led to the precipitation of soap and part of non-P3HB cell mass. The resulting precipitate was utilized as a carbon source in biomass production and increased substrate-to-P3HB conversion.

Keywords

PHA; P3HB; Recovery; Downstream process; Isolation

Key words in English

PHA; P3HB; Recovery; Downstream process; Isolation

Authors

POSPÍŠILOVÁ, A.; NOVÁČKOVÁ, I.; PŘIKRYL, R.

RIV year

2021

Released

01.04.2021

Publisher

Elsevier

Location

OXFORD

ISBN

0960-8524

Periodical

BIORESOURCE TECHNOLOGY

Volume

326

Number

1

State

Kingdom of the Netherlands

Pages from

1

Pages to

5

Pages count

5

URL

https://www.sciencedirect.com/science/article/pii/S0960852421000213

Full text in the Digital Library

http://hdl.handle.net/11012/203003

BibTex

@article{BUT171646,
  author="Aneta {Pospíšilová} and Ivana {Nováčková} and Radek {Přikryl}",
  title="Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil",
  journal="BIORESOURCE TECHNOLOGY",
  year="2021",
  volume="326",
  number="1",
  pages="1--5",
  doi="10.1016/j.biortech.2021.124683",
  issn="0960-8524",
  url="https://www.sciencedirect.com/science/article/pii/S0960852421000213"
}

Documents

PHB_isolation_using_soap_modified_bib_style
124683_accepted