Publication result detail

Determining spatial distribution of interleukin-1 beta as an infection marker in pulmonary porcine tissues

JAROŠOVÁ, R.; DO, T.; TESAŘOVÁ, B.; ŠMÍDOVÁ, V.; GURÁŇ, R.; ONDRÁČKOVÁ, P.; FALDYNA, M.; SLÁDEK, Z.; ZÍTKA, O.

Original Title

Determining spatial distribution of interleukin-1 beta as an infection marker in pulmonary porcine tissues

English Title

Determining spatial distribution of interleukin-1 beta as an infection marker in pulmonary porcine tissues

Type

Paper in proceedings (conference paper)

Original Abstract

Interleukin-1 beta (IL-1 beta) belongs among proinflammatory cytokines that are produced by activated macrophages as an immune response of the organism. Therefore, IL-1 beta could serve as a possible biomarker of a bacterial infection caused by Actinobacillus pleuropneumonia. In this study, we applied matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) together with immunohistochemical analyses (IHC) using primary antibody anti-IL-1 beta for IL-1 beta detection in cryo-sectioned pulmonary tissues. Using MALDI MSI, we detected and spatially visualized the tryptic fragment of IL-1 beta. This IL-1 beta tryptic fragment was more frequently detected in infected pulmonary tissues compared to healthy pulmonary tissue except one necrotic lung tissue where was not revealed the statistically significant difference. IHC confirmed the occurrence of IL-1 beta in necrotic pulmonary tissues. This knowledge could be useful for further clinical studying as a targeted therapy.

English abstract

Interleukin-1 beta (IL-1 beta) belongs among proinflammatory cytokines that are produced by activated macrophages as an immune response of the organism. Therefore, IL-1 beta could serve as a possible biomarker of a bacterial infection caused by Actinobacillus pleuropneumonia. In this study, we applied matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) together with immunohistochemical analyses (IHC) using primary antibody anti-IL-1 beta for IL-1 beta detection in cryo-sectioned pulmonary tissues. Using MALDI MSI, we detected and spatially visualized the tryptic fragment of IL-1 beta. This IL-1 beta tryptic fragment was more frequently detected in infected pulmonary tissues compared to healthy pulmonary tissue except one necrotic lung tissue where was not revealed the statistically significant difference. IHC confirmed the occurrence of IL-1 beta in necrotic pulmonary tissues. This knowledge could be useful for further clinical studying as a targeted therapy.

Keywords

immunohistochemistry; mass spectrometry imaging; infection; cytokine; interleukin; pig model; IL-1 beta

Key words in English

immunohistochemistry; mass spectrometry imaging; infection; cytokine; interleukin; pig model; IL-1 beta

Authors

JAROŠOVÁ, R.; DO, T.; TESAŘOVÁ, B.; ŠMÍDOVÁ, V.; GURÁŇ, R.; ONDRÁČKOVÁ, P.; FALDYNA, M.; SLÁDEK, Z.; ZÍTKA, O.

RIV year

2021

Released

07.11.2019

ISBN

978-80-7509-688-3

Book

MendelNet 2019: Proceedings of International PhD Students Conference

Pages from

459

Pages to

464

Pages count

6

URL

https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf

BibTex

@inproceedings{BUT170488,
  author="Rea {Jarošová} and Tomáš {Do} and Barbora {Tesařová} and Veronika {Šmídová} and Roman {Guráň} and Petra {Ondráčková} and Martin {Faldyna} and Zbyšek {Sládek} and Ondřej {Zítka}",
  title="Determining spatial distribution of interleukin-1 beta as an infection marker in pulmonary porcine tissues",
  booktitle="MendelNet 2019: Proceedings of International PhD Students Conference",
  year="2019",
  pages="459--464",
  isbn="978-80-7509-688-3",
  url="https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf"
}