Publication result detail

Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

Pilat, Zdenek; Jezek, Jan; Kizovsky, Martin; Klementova, Tereza; Kratky, Stanislav; Sobota, Jaroslav; Samek, Ota; Zemanek, Pavel; Buryska, Tomas; Damborsky, Jiri; Prokop, Zbynek

Original Title

Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

English Title

Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

Type

WoS Article

Original Abstract

Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.

English abstract

Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.

Keywords

Substrates; Springs; Raman scattering; Photonics; Electromagnetics; Measurement by laser beam; Gold

Key words in English

Substrates; Springs; Raman scattering; Photonics; Electromagnetics; Measurement by laser beam; Gold

Authors

Pilat, Zdenek; Jezek, Jan; Kizovsky, Martin; Klementova, Tereza; Kratky, Stanislav; Sobota, Jaroslav; Samek, Ota; Zemanek, Pavel; Buryska, Tomas; Damborsky, Jiri; Prokop, Zbynek

RIV year

2021

Released

02.03.2020

Publisher

IEEE

Location

NEW YORK

ISBN

1559-9450

Book

2019 PhotonIcs & Electromagnetics Research Symposium - Spring (PIERS-Spring)

ISBN

1559-9450

Periodical

Progress In Electromagnetics

Volume

2019

Number

1

State

United States of America

Pages from

1301

Pages to

1309

Pages count

9

URL

https://ieeexplore-ieee-org.ezproxy.lib.vutbr.cz/document/9017521

BibTex

@article{BUT170289,
  author="Pilat, Zdenek and Jezek, Jan and Kizovsky, Martin and Klementova, Tereza and Kratky, Stanislav and Sobota, Jaroslav and Samek, Ota and Zemanek, Pavel and Buryska, Tomas and Damborsky, Jiri and Prokop, Zbynek",
  title="Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring",
  journal="Progress In Electromagnetics",
  year="2020",
  volume="2019",
  number="1",
  pages="1301--1309",
  doi="10.1109/PIERS-Spring46901.2019.9017521",
  issn="1559-9450",
  url="https://ieeexplore-ieee-org.ezproxy.lib.vutbr.cz/document/9017521"
}