Publication result detail

Newly designed primers targeting limonene synthase

ROMANOVSKÁ, D.; FIALOVÁ, L.; MÁROVÁ, I.

Original Title

Newly designed primers targeting limonene synthase

English Title

Newly designed primers targeting limonene synthase

Type

Abstract

Original Abstract

Cosmetic products with ingredients of plant origin promise beneficial effects on the customer health. Those may contain allergens that belong to the group of 24 fragrance allergens that can negatively affect the health of sensitive individuals. One of these allergens is d-limonene present for example in citrus fruits. The presence of plant DNA in the cosmetic product can be detected using techniques of molecular biotechnology, even if it is present in very small quantity. New primers Lim2 F/R, targeting limonene synthase, were designed to confirm the presence of the citrus DNA in a leave-on cosmetic product. The DNA of Citrus limon was isolated from the leave-on cosmetic product and the specific sequence was amplified using real-time PCR. The presence of amplicons of specific size was confirmed using agarose electrophoresis.

English abstract

Cosmetic products with ingredients of plant origin promise beneficial effects on the customer health. Those may contain allergens that belong to the group of 24 fragrance allergens that can negatively affect the health of sensitive individuals. One of these allergens is d-limonene present for example in citrus fruits. The presence of plant DNA in the cosmetic product can be detected using techniques of molecular biotechnology, even if it is present in very small quantity. New primers Lim2 F/R, targeting limonene synthase, were designed to confirm the presence of the citrus DNA in a leave-on cosmetic product. The DNA of Citrus limon was isolated from the leave-on cosmetic product and the specific sequence was amplified using real-time PCR. The presence of amplicons of specific size was confirmed using agarose electrophoresis.

Keywords

Cosmetics, plant DNA, Citrus limon, primers, PCR

Key words in English

Cosmetics, plant DNA, Citrus limon, primers, PCR

Authors

ROMANOVSKÁ, D.; FIALOVÁ, L.; MÁROVÁ, I.

RIV year

2021

Released

26.02.2021

Publisher

Taylor and Francis

Location

Prague

ISBN

1310-2818

Book

Biotechnology & Biotechnological Equipment, Special Issue:European Biotechnology Congress 2020

Edition

35

ISBN

1314-3530

Periodical

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT

Volume

35

Number

Sup 1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

119

Pages to

119

Pages count

1

URL

https://www.tandfonline.com/doi/full/10.1080/13102818.2020.1871545?fbclid=IwAR0gLYRgommM-eySZC1qddUErA0VWyAlsj5S0gYm3QG8A0HLbrOJyJyu624

BibTex

@misc{BUT170131,
  author="Denisa {Langová} and Lenka {Fialová} and Ivana {Márová}",
  title="Newly designed primers targeting limonene synthase",
  booktitle="Biotechnology & Biotechnological Equipment, Special Issue:European Biotechnology Congress 2020",
  year="2021",
  series="35",
  edition="sup1",
  journal="BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT",
  volume="35",
  number="Sup 1",
  pages="119--119",
  publisher="Taylor and Francis",
  address="Prague",
  isbn="1310-2818",
  issn="1310-2818",
  url="https://www.tandfonline.com/doi/full/10.1080/13102818.2020.1871545?fbclid=IwAR0gLYRgommM-eySZC1qddUErA0VWyAlsj5S0gYm3QG8A0HLbrOJyJyu624",
  note="Abstract"
}