Publication detail

Image-Based Motion Correction for Optical Mapping of Cardiac Electrical Activity

KHWAOUNJOO, P. RUTHERFORD, S. ŠVRČEK, M. LEGRICE, I. TREW, M. SMAILL, B.

Original Title

Image-Based Motion Correction for Optical Mapping of Cardiac Electrical Activity

Type

journal article in Web of Science

Language

English

Original Abstract

Optical mapping, with membrane-bound, voltage-sensitive dyes, is widely used for in vitro recording of cardiac electrical activity. The spatial registration of such maps is lost when the heart moves with respect to a fixed photodetector array and contraction can generate substantial artifact if background fluorescence is not uniformly distributed. While motion artifact is commonly suppressed with electromechanical uncoupling agents, there are circumstances where these are undesirable. This study outlines a novel image-based approach for retrospective motion artifact correction. Isolated Langendorff-supported rat hearts (n = 8), stained with di-4-ANEPPS, were illuminated at 516 +/- A 14 nm and fluorescent emission (> 565 +/- A 10 nm) was acquired with a charge multiplying CCD camera. Background fluorescence was segmented in successive frames and stabilized using a non-rigid image registration algorithm. The resultant image deformation was used to estimate material point movement on the heart surface, so that total fluorescence could be mapped frame-by-frame to appropriate reference pixels. Finally, residual motion artifact was identified and removed. The effectiveness of this correction method was evaluated over 18 experimental datasets. Signal-to-noise ratio was increased more than fourfold, and activation time and action potential duration (APD) could be estimated at 24% more pixels than in the raw data. The variability of all APD measures was substantially reduced (i.e. APD50 estimated as 83.8 +/- A 45.8 ms before correction was 52.1 +/- A 4.7 ms afterward). This approach provides a robust means of recovering optical action potentials in the presence of substantial motion artifact.

Keywords

Optical mapping; Cardiac; Electrical activation; Motion correction; Active demons algorithm; Electrophysiology

Authors

KHWAOUNJOO, P.; RUTHERFORD, S.; ŠVRČEK, M.; LEGRICE, I.; TREW, M.; SMAILL, B.

Released

11. 11. 2014

Publisher

SPRINGER

Location

NEW YORK

ISBN

1573-9686

Periodical

ANNALS OF BIOMEDICAL ENGINEERING

Year of study

43

Number

5

State

United States of America

Pages from

1235

Pages to

1246

Pages count

12

URL

https://link.springer.com/article/10.1007%2Fs10439-014-1172-8

BibTex

@article{BUT169896,
  author="Prashanna {Khwaounjoo} and Sally {Rutherford} and Martin {Švrček} and Ian {LeGrice} and Mark {Trew} and Bruce {Smaill}",
  title="Image-Based Motion Correction for Optical Mapping of Cardiac Electrical Activity",
  journal="ANNALS OF BIOMEDICAL ENGINEERING",
  year="2014",
  volume="43",
  number="5",
  pages="1235--1246",
  doi="10.1007/s10439-014-1172-8",
  issn="1573-9686",
  url="https://link.springer.com/article/10.1007%2Fs10439-014-1172-8"
}