Publication result detail

G-quadruplexes in H1N1 influenza genomes

BRÁZDA, V.; PORUBIAKOVÁ, O.; CANTARA, A.; BOHÁLOVÁ, N.; COUFAL, J.; BARTAS, M.; FOJTA, M.; MERGNY, J.

Original Title

G-quadruplexes in H1N1 influenza genomes

English Title

G-quadruplexes in H1N1 influenza genomes

Type

WoS Article

Original Abstract

Background: Influenza viruses are dangerous pathogens. Seventy-Seven genomes of recently emerged genotype 4 reassortant Eurasian avian-like H1N1 virus (G4-EA-H1N1) are currently available. We investigated the presence and variation of potential G-quadruplex forming sequences (PQS), which can serve as targets for antiviral treatment. Results: PQS were identified in all 77 genomes. The total number of PQS in G4-EA-H1N1 genomes was 571. Interestingly, the number of PQS per genome in individual close relative viruses varied from 4 to 12. PQS were not randomly distributed in the 8 segments of the G4-EA-H1N1 genome, the highest frequency of PQS being found in the NP segment (1.39 per 1000 nt), which is considered a potential target for antiviral therapy. In contrast, no PQS was found in the NS segment. Analyses of variability pointed the importance of some PQS; even if genome variation of influenza virus is extreme, the PQS with the highest G4Hunter score is the most conserved in all tested genomes. G-quadruplex formation in vitro was experimentally confirmed using spectroscopic methods. Conclusions: The results presented here hint several G-quadruplex-forming sequences in G4-EA-H1N1 genomes, that could provide good therapeutic targets.

English abstract

Background: Influenza viruses are dangerous pathogens. Seventy-Seven genomes of recently emerged genotype 4 reassortant Eurasian avian-like H1N1 virus (G4-EA-H1N1) are currently available. We investigated the presence and variation of potential G-quadruplex forming sequences (PQS), which can serve as targets for antiviral treatment. Results: PQS were identified in all 77 genomes. The total number of PQS in G4-EA-H1N1 genomes was 571. Interestingly, the number of PQS per genome in individual close relative viruses varied from 4 to 12. PQS were not randomly distributed in the 8 segments of the G4-EA-H1N1 genome, the highest frequency of PQS being found in the NP segment (1.39 per 1000 nt), which is considered a potential target for antiviral therapy. In contrast, no PQS was found in the NS segment. Analyses of variability pointed the importance of some PQS; even if genome variation of influenza virus is extreme, the PQS with the highest G4Hunter score is the most conserved in all tested genomes. G-quadruplex formation in vitro was experimentally confirmed using spectroscopic methods. Conclusions: The results presented here hint several G-quadruplex-forming sequences in G4-EA-H1N1 genomes, that could provide good therapeutic targets.

Keywords

Influenza virus, G-quadruplex, G4Hunter

Key words in English

Influenza virus, G-quadruplex, G4Hunter

Authors

BRÁZDA, V.; PORUBIAKOVÁ, O.; CANTARA, A.; BOHÁLOVÁ, N.; COUFAL, J.; BARTAS, M.; FOJTA, M.; MERGNY, J.

RIV year

2021

Released

24.01.2021

Publisher

Springer Nature

Location

London,UK

ISBN

1471-2164

Periodical

BMC GENOMICS

Volume

22

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

11

Pages count

11

URL

https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-07377-9

Full text in the Digital Library

http://hdl.handle.net/11012/196699

BibTex

@article{BUT168095,
  author="Václav {Brázda} and Otília {Porubiaková} and Alessio {Cantara} and Natália {Bohálová} and Jan {Coufal} and Martin {Bartas} and Miroslav {Fojta} and Jean-Louis {Mergny}",
  title="G-quadruplexes in H1N1 influenza genomes",
  journal="BMC GENOMICS",
  year="2021",
  volume="22",
  number="1",
  pages="1--11",
  doi="10.1186/s12864-021-07377-9",
  issn="1471-2164",
  url="https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-07377-9"
}

Documents

s12864-021-07377-9