Detail publikačního výsledku

Phenotypic and genomic analysis of isopropanol and 1,3-propanediol producer Clostridium diolis DSM 15410

SEDLÁŘ, K.; VASYLKIVSKA, M.; MUSILOVÁ, J.; BRANSKÁ, B.; PROVAZNÍK, I.; PATÁKOVÁ, P.

Original Title

Phenotypic and genomic analysis of isopropanol and 1,3-propanediol producer Clostridium diolis DSM 15410

English Title

Phenotypic and genomic analysis of isopropanol and 1,3-propanediol producer Clostridium diolis DSM 15410

Type

WoS Article

Original Abstract

Clostridium diolis DSM 15410 is a type strain of solventogenic clostridium capable of conducting isopropanol-butanol-ethanol fermentation. By studying its growth on different carbohydrates, we verified its ability to utilize glycerol and produce 1,3-propanediol and discovered its ability to produced isopropanol. Complete genome sequencing showed that its genome is a single circular chromosome and belongs to the cluster I (sensu scricto) of the genus Clostridium. By cultivation analysis we highlighted its specific behavior in comparison to two selected closely related strains. Despite the fact that several CRISPR loci were found, 16 putative prophages showed the ability to receive foreign DNA. Thus, the strain has the necessary features for future engineering of its 1,3-propanediol biosynthetic pathway and for the possible industrial utilization in the production of biofuels.

English abstract

Clostridium diolis DSM 15410 is a type strain of solventogenic clostridium capable of conducting isopropanol-butanol-ethanol fermentation. By studying its growth on different carbohydrates, we verified its ability to utilize glycerol and produce 1,3-propanediol and discovered its ability to produced isopropanol. Complete genome sequencing showed that its genome is a single circular chromosome and belongs to the cluster I (sensu scricto) of the genus Clostridium. By cultivation analysis we highlighted its specific behavior in comparison to two selected closely related strains. Despite the fact that several CRISPR loci were found, 16 putative prophages showed the ability to receive foreign DNA. Thus, the strain has the necessary features for future engineering of its 1,3-propanediol biosynthetic pathway and for the possible industrial utilization in the production of biofuels.

Keywords

Clostridium diolis; complete genome; solventogenic; IBE; 1,3-propanediol

Key words in English

Clostridium diolis; complete genome; solventogenic; IBE; 1,3-propanediol

Authors

SEDLÁŘ, K.; VASYLKIVSKA, M.; MUSILOVÁ, J.; BRANSKÁ, B.; PROVAZNÍK, I.; PATÁKOVÁ, P.

RIV year

2021

Released

07.11.2020

Publisher

Elsevier

ISBN

0888-7543

Periodical

GENOMICS

Volume

113

Number

1

State

United States of America

Pages from

1109

Pages to

1119

Pages count

11

URL

https://www.sciencedirect.com/science/article/pii/S088875432032005X

Full text in the Digital Library

http://hdl.handle.net/11012/196477

BibTex

@article{BUT165852,
  author="Karel {Sedlář} and Maryna {Vasylkivska} and Jana {Musilová} and Barbora {Branská} and Valentýna {Provazník} and Petra {Patáková}",
  title="Phenotypic and genomic analysis of isopropanol and 1,3-propanediol producer Clostridium diolis DSM 15410",
  journal="GENOMICS",
  year="2020",
  volume="113",
  number="1",
  pages="1109--1119",
  doi="10.1016/j.ygeno.2020.11.007",
  issn="0888-7543",
  url="https://www.sciencedirect.com/science/article/pii/S088875432032005X"
}

Documents

GENO_2020_1304_R1
GENO_2020_1304_accepted