Potential role of compost mixed biochar with rhizobacteria in mitigating lead toxicity in spinach

Potential role of compost mixed biochar with rhizobacteria in mitigating lead toxicity in spinach

WoS Article

Consumption of heavy metals, especially lead (Pb) contaminated food is a serious threat to human health. Higher Pb uptake by the plant affects the quality, growth and yield of crops. However, inoculation of plant growth-promoting rhizobacteria (PGPR) along with a mixture of organic amendments and biochar could be an effective way to overcome the problem of Pb toxicity. That's why current pot experiment was conducted to investigate the effect of compost mixed biochar (CB) and ACC deaminase producing PGPR on growth and yield of spinach plants under artificially induced Pb toxicity. Six different treatments i.e., control, Alcaligenes faecalis (PGPR1), Bacillus amyloliquefaciens (PGPR2), compost+biochar (CB), PGPR1+CB and PGPR2+CB were applied under 250 mg Pb kg(-1) soil. Results showed that inoculation of PGPRs (Alcaligenes faecalis and Bacillus amyloliquefaciens) alone and along with CB significantly enhanced root fresh (47%) and dry weight (31%), potassium concentration (11%) in the spinach plant. Whereas, CB+Bacillus amyloliquefaciens significantly decreased (43%) the concentration of Pb in the spinach root over control. In conclusion, CB+Bacillus amyloliquefaciens has the potential to mitigate the Pb induced toxicity in the spinach. The obtained result can be further used in the planning and execution of rhizobacteria and compost mixed biochar-based soil amendment.

Consumption of heavy metals, especially lead (Pb) contaminated food is a serious threat to human health. Higher Pb uptake by the plant affects the quality, growth and yield of crops. However, inoculation of plant growth-promoting rhizobacteria (PGPR) along with a mixture of organic amendments and biochar could be an effective way to overcome the problem of Pb toxicity. That's why current pot experiment was conducted to investigate the effect of compost mixed biochar (CB) and ACC deaminase producing PGPR on growth and yield of spinach plants under artificially induced Pb toxicity. Six different treatments i.e., control, Alcaligenes faecalis (PGPR1), Bacillus amyloliquefaciens (PGPR2), compost+biochar (CB), PGPR1+CB and PGPR2+CB were applied under 250 mg Pb kg(-1) soil. Results showed that inoculation of PGPRs (Alcaligenes faecalis and Bacillus amyloliquefaciens) alone and along with CB significantly enhanced root fresh (47%) and dry weight (31%), potassium concentration (11%) in the spinach plant. Whereas, CB+Bacillus amyloliquefaciens significantly decreased (43%) the concentration of Pb in the spinach root over control. In conclusion, CB+Bacillus amyloliquefaciens has the potential to mitigate the Pb induced toxicity in the spinach. The obtained result can be further used in the planning and execution of rhizobacteria and compost mixed biochar-based soil amendment.

GROWTH-PROMOTING RHIZOBACTERIA; WHEAT TRITICUM-AESTIVUM; PLANT-GROWTH; ACC-DEAMINASE; CARBON MINERALIZATION; NUTRIENT-UPTAKE; BLACK CARBON; SOIL; NITROGEN; BACTERIA

GROWTH-PROMOTING RHIZOBACTERIA; WHEAT TRITICUM-AESTIVUM; PLANT-GROWTH; ACC-DEAMINASE; CARBON MINERALIZATION; NUTRIENT-UPTAKE; BLACK CARBON; SOIL; NITROGEN; BACTERIA

Muhammad Zafar‑ul‑Hye; Muhammad tahzeeb‑ul‑Hassan; Muhammad Abid; Shah Fahad; Martin Brtnicky; Tereza Dokulilova; Rahul Datta; Subhan Danish

2021

22.07.2020

NATURE PUBLISHING GROUP

LONDON

2045-2322

Scientific Reports

10

12159

United Kingdom of Great Britain and Northern Ireland

1

12

12

https://www.nature.com/articles/s41598-020-69183-9