Publication result detail

Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography

KUNISHIMA, N.; TAKEDA, Y.; HIROSE, R.; KALASOVÁ, D.; ŠALPLACHTA, J., OMOTE, K.

Original Title

Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography

English Title

Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography

Type

WoS Article

Original Abstract

The visualization of internal 3D-structure of tissues at micron resolutions without staining by contrast reagents is desirable in plant researches, and it can be achieved by an X-ray computed tomography (CT) with a phase-retrieval technique. Recently, a laboratory-based X-ray microscope adopting the phase contrast CT was developed as a powerful tool for the observation of weakly absorbing biological samples. Here we report the observation of unstained pansy seeds using the laboratory-based X-ray phase-contrast CT.

English abstract

The visualization of internal 3D-structure of tissues at micron resolutions without staining by contrast reagents is desirable in plant researches, and it can be achieved by an X-ray computed tomography (CT) with a phase-retrieval technique. Recently, a laboratory-based X-ray microscope adopting the phase contrast CT was developed as a powerful tool for the observation of weakly absorbing biological samples. Here we report the observation of unstained pansy seeds using the laboratory-based X-ray phase-contrast CT.

Keywords

3D rendering; Germination; Pansy seeds; Tomography; X-ray microscope

Key words in English

3D rendering; Germination; Pansy seeds; Tomography; X-ray microscope

Authors

KUNISHIMA, N.; TAKEDA, Y.; HIROSE, R.; KALASOVÁ, D.; ŠALPLACHTA, J., OMOTE, K.

RIV year

2021

Released

01.02.2020

Publisher

BioMed Central

ISBN

1746-4811

Periodical

Plant Methods

Volume

16

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

10

Pages count

10

URL

http://link.springer.com/article/10.1186/s13007-020-0557-y

Full text in the Digital Library

http://hdl.handle.net/11012/193426

BibTex

@article{BUT161932,
  author="Naoki {Kunishima} and Yoshihiro {Takeda} and Raita {Hirose} and Dominika {Kalasová} and Jakub {Šalplachta} and Kazuhiko {Omote}",
  title="Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography",
  journal="Plant Methods",
  year="2020",
  volume="16",
  number="1",
  pages="1--10",
  doi="10.1186/s13007-020-0557-y",
  url="http://link.springer.com/article/10.1186/s13007-020-0557-y"
}

Documents

13007-020-0557-y