Publication detail

Appropriate techniques of structural characterization of a model hydrogels system

TRUDIČOVÁ, M. SEDLÁČEK, P. KRZYŽÁNEK, V. ADÁMKOVÁ, K.

Original Title

Appropriate techniques of structural characterization of a model hydrogels system

English Title

Appropriate techniques of structural characterization of a model hydrogels system

Type

abstract

Language

en

Original Abstract

The main focus of this work was testing the applicability of available structural analysis techniques on an appropriate model hydrogel system and assessing the influence of the composition change of the hydrogel system on its internal structure. Semi-interpenetrating network hydrogels based on combination of agarose and sodium poly(styrene sulfonate) of various concentrations were chosen as a model system. Reference gels of four agarose concentrations were studied (0.5 %; 1.0 %; 2.0 % and 4.0 %) and compared with the gels with fixed concentration of agarose (1 wt. %) interpenetrated by polystyrene sulfonate at various concentrations (0.002 %; 0.005 % and 0.01 %). Swelled (hydrated) forms of the gels are usually used for applications, so it is convenient to examine samples in this state; however, there are not many common and available methods suitable for examining the structure of hydrated samples. Dry samples show artifacts in their structure due to eliminating water, e.g. structure of freeze-dried samples is affected by water freezing and subsequent crystal growth. By optimizing the drying process, this effect can be minimized. For dry (shock-frozen in liquid nitrogen and vacuum freeze-dried) samples, mercury porosimetry as an indirect method and scanning electron microscopy (SEM) as a direct method were selected in this study. For native (swelled) samples, turbidimetry as an indirect method and cryo SEM as a direct method were performed.

English abstract

The main focus of this work was testing the applicability of available structural analysis techniques on an appropriate model hydrogel system and assessing the influence of the composition change of the hydrogel system on its internal structure. Semi-interpenetrating network hydrogels based on combination of agarose and sodium poly(styrene sulfonate) of various concentrations were chosen as a model system. Reference gels of four agarose concentrations were studied (0.5 %; 1.0 %; 2.0 % and 4.0 %) and compared with the gels with fixed concentration of agarose (1 wt. %) interpenetrated by polystyrene sulfonate at various concentrations (0.002 %; 0.005 % and 0.01 %). Swelled (hydrated) forms of the gels are usually used for applications, so it is convenient to examine samples in this state; however, there are not many common and available methods suitable for examining the structure of hydrated samples. Dry samples show artifacts in their structure due to eliminating water, e.g. structure of freeze-dried samples is affected by water freezing and subsequent crystal growth. By optimizing the drying process, this effect can be minimized. For dry (shock-frozen in liquid nitrogen and vacuum freeze-dried) samples, mercury porosimetry as an indirect method and scanning electron microscopy (SEM) as a direct method were selected in this study. For native (swelled) samples, turbidimetry as an indirect method and cryo SEM as a direct method were performed.

Keywords

hydrogel, structural analysis, electron microscopy, mercury porosimetry, turbidimetry

Released

21.11.2019

Publisher

Vysoké učení technické v Brně, Fakulta chemická

Location

Brno

ISBN

978-80-214-5807-9

Book

Studentská odborná konference Chemie je život 2019 - Sborník abstraktů

Edition number

1

Pages from

87

Pages to

88

Pages count

2

Documents

BibTex


@misc{BUT161327,
  author="Monika {Trudičová} and Petr {Sedláček} and Vladislav {Krzyžánek}",
  title="Appropriate techniques of structural characterization of a model hydrogels system",
  annote="The main focus of this work was testing the applicability of available structural analysis techniques on an appropriate model hydrogel system and assessing the influence of the composition change of the hydrogel system on its internal structure. Semi-interpenetrating network hydrogels based on combination of agarose and sodium poly(styrene sulfonate) of various concentrations were chosen as a model system. Reference gels of four agarose concentrations were studied (0.5 %; 1.0 %; 2.0 % and 4.0 %) and compared with the gels with fixed concentration of agarose (1 wt. %) interpenetrated by polystyrene sulfonate at various concentrations (0.002 %; 0.005 % and 0.01 %). Swelled (hydrated) forms of the gels are usually used for applications, so it is convenient to examine samples in this state; however, there are not many common and available methods suitable for examining the structure of hydrated samples. Dry samples show artifacts in their structure due to eliminating water, e.g. structure of freeze-dried samples is affected by water freezing and subsequent crystal growth. By optimizing the drying process, this effect can be minimized. For dry (shock-frozen in liquid nitrogen and vacuum freeze-dried) samples, mercury porosimetry as an indirect method and scanning electron microscopy (SEM) as a direct method were selected in this study. For native (swelled) samples, turbidimetry as an indirect method and cryo SEM as a direct method were performed.",
  address="Vysoké učení technické v Brně, Fakulta chemická",
  booktitle="Studentská odborná konference Chemie je život 2019 - Sborník abstraktů",
  chapter="161327",
  howpublished="print",
  institution="Vysoké učení technické v Brně, Fakulta chemická",
  year="2019",
  month="november",
  pages="87--88",
  publisher="Vysoké učení technické v Brně, Fakulta chemická",
  type="abstract"
}