Publication result detail

Electron Spin Resonance Detection of Human MicroRNA 21-5p in Renal Cell Carcinoma

HRUBÝ, J.; KOVÁČOVÁ, J; SLABÝ, O; NEUGEBAUER, P.

Original Title

Electron Spin Resonance Detection of Human MicroRNA 21-5p in Renal Cell Carcinoma

English Title

Electron Spin Resonance Detection of Human MicroRNA 21-5p in Renal Cell Carcinoma

Type

Abstract

Original Abstract

Our approach aims at applying the electron spin resonance (ESR) spectroscopy to a complex biological system and establishing a semi-invasive method of detection of human microRNA (miRNA) in renal cell carcinoma. Nowadays, detection of miRNAs´ presence in cancer cells is done by performing quantitative polymerase chain reaction (qPCR) which monitors gene amplification of a target sequence by attaching fluorescent chemical compounds. Herein, we are focused on gaining an insight into the physical and chemical environment by using a spin probe, namely a nitroxide spin label (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl (TEMPO) directly attached to anti-hsa-miR-21-5p oligonucleotides.

English abstract

Our approach aims at applying the electron spin resonance (ESR) spectroscopy to a complex biological system and establishing a semi-invasive method of detection of human microRNA (miRNA) in renal cell carcinoma. Nowadays, detection of miRNAs´ presence in cancer cells is done by performing quantitative polymerase chain reaction (qPCR) which monitors gene amplification of a target sequence by attaching fluorescent chemical compounds. Herein, we are focused on gaining an insight into the physical and chemical environment by using a spin probe, namely a nitroxide spin label (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl (TEMPO) directly attached to anti-hsa-miR-21-5p oligonucleotides.

Keywords

electron spin resonance, microRNA, spin label

Key words in English

electron spin resonance, microRNA, spin label

Authors

HRUBÝ, J.; KOVÁČOVÁ, J; SLABÝ, O; NEUGEBAUER, P.

Released

25.06.2019

Publisher

Masarykova Univerzita

Location

Brno

ISBN

978-80-210-9300-3

Book

Joint Retreat 2019

Pages from

53

Pages to

53

Pages count

156

URL

http://phdretreat.ceitec.cz/

BibTex

@misc{BUT157611,
  author="HRUBÝ, J. and KOVÁČOVÁ, J and SLABÝ, O and NEUGEBAUER, P.",
  title="Electron Spin Resonance Detection of Human MicroRNA 21-5p in Renal Cell Carcinoma",
  booktitle="Joint Retreat 2019",
  year="2019",
  pages="53--53",
  publisher="Masarykova Univerzita",
  address="Brno",
  isbn="978-80-210-9300-3",
  url="http://phdretreat.ceitec.cz/",
  note="Abstract"
}