Publication result detail

Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing

VANĚČKOVÁ, T.; POMPEIANO VANÍČKOVÁ, L.; TVRDOŇOVÁ, M.; POMORSKI, A.; KRĘŻEL, A.; VACULOVIČ, T.; KANICKÝ, V.; VACULOVIČOVÁ, M.; ADAM, V.

Original Title

Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing

English Title

Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing

Type

WoS Article

Original Abstract

We report a facile method for detection of metallothionein (MT), a promising clinically relevant biomarker, in spiked plasma samples. This method, for the first time, integrates molecularly imprinted polymers as purification/pretreatment step with matrix assisted laser desorption/ionization time-of-flight mass spectrometric detection and with laser ablation inductively coupled plasma mass spectrometry for analysis of MTs. The prepared MT-imprinted polydopamine layer showed high binding capacity and specific recognition properties toward the template. Optimal monomer (dopamine) concentration was found to be 16 mM of dopamine. This experimental setup allows to measure mu M concentrations of MT that are present in blood as this can be used for clinical studies recognizing MT as marker of various diseases including tumour one. Presented approach not only provides fast sample throughput but also avoids the limitations of methods based on use of antibodies (e.g. high price, cross reactivity, limited availability in some cases, etc.).

English abstract

We report a facile method for detection of metallothionein (MT), a promising clinically relevant biomarker, in spiked plasma samples. This method, for the first time, integrates molecularly imprinted polymers as purification/pretreatment step with matrix assisted laser desorption/ionization time-of-flight mass spectrometric detection and with laser ablation inductively coupled plasma mass spectrometry for analysis of MTs. The prepared MT-imprinted polydopamine layer showed high binding capacity and specific recognition properties toward the template. Optimal monomer (dopamine) concentration was found to be 16 mM of dopamine. This experimental setup allows to measure mu M concentrations of MT that are present in blood as this can be used for clinical studies recognizing MT as marker of various diseases including tumour one. Presented approach not only provides fast sample throughput but also avoids the limitations of methods based on use of antibodies (e.g. high price, cross reactivity, limited availability in some cases, etc.).

Keywords

Metallothionein; Molecularly imprinted polymers; Polydopamine; MALDI-TOP-MS; LA-ICP-MS

Key words in English

Metallothionein; Molecularly imprinted polymers; Polydopamine; MALDI-TOP-MS; LA-ICP-MS

Authors

VANĚČKOVÁ, T.; POMPEIANO VANÍČKOVÁ, L.; TVRDOŇOVÁ, M.; POMORSKI, A.; KRĘŻEL, A.; VACULOVIČ, T.; KANICKÝ, V.; VACULOVIČOVÁ, M.; ADAM, V.

RIV year

2019

Released

01.06.2019

Publisher

Elsevier

ISBN

0039-9140

Periodical

TALANTA

Volume

198

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

224

Pages to

229

Pages count

6

URL

https://www.sciencedirect.com/science/article/pii/S0039914019301109

Full text in the Digital Library

http://hdl.handle.net/11012/195645

BibTex

@article{BUT156653,
  author="Tereza {Vaněčková} and Lucie {Pompeiano Vaníčková} and Michaela {Kuchynka} and Adam {Pomorski} and Artur {Krężel} and Tomáš {Vaculovič} and Viktor {Kanický} and Markéta {Vaculovičová} and Vojtěch {Adam}",
  title="Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing",
  journal="TALANTA",
  year="2019",
  volume="198",
  number="1",
  pages="224--229",
  doi="10.1016/j.talanta.2019.01.089",
  issn="0039-9140",
  url="https://www.sciencedirect.com/science/article/pii/S0039914019301109"
}

Documents

Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing
molecularly-imprinted-vut