Publication result detail

Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(a-amino acids)

SEDLAČÍK, T.; ACAR, O.; STUDENOVSKÁ, H.; KOTELNIKOV, I.; KUČKA, J.; KONEČNÁ, Z.; ZIKMUND, T.; KAISER, J.; KOSE, G.; RYPÁČEK, F.

Original Title

Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(a-amino acids)

English Title

Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(a-amino acids)

Type

WoS Article

Original Abstract

In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(a-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-L-glutamine-stat-N5-(2-methacryloyl-oxyethyl)- L-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers. PrMAAm provided alkyne groups for modifying the gels with cellsupporting moieties (RGDS peptides) by the azide–alkyne ‘‘click’’-reaction. Two types of gels with different compressive moduli were prepared. Each type was modified with two different concentrations of RGDS peptide. X-ray computed nanotomography (nanoCT) was used to visualize and analyze the 3D-structure of the cryogels. It was shown that modifying the PHEG-MA cryogels within the range of RGDS concentrations examined here had a positive effect on the proliferation of hDPSCs. Immunofluorescence staining for collagen type 2 and aggrecan proved that there was differentiation of hDPSCs into chondrocytes.

English abstract

In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(a-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-L-glutamine-stat-N5-(2-methacryloyl-oxyethyl)- L-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers. PrMAAm provided alkyne groups for modifying the gels with cellsupporting moieties (RGDS peptides) by the azide–alkyne ‘‘click’’-reaction. Two types of gels with different compressive moduli were prepared. Each type was modified with two different concentrations of RGDS peptide. X-ray computed nanotomography (nanoCT) was used to visualize and analyze the 3D-structure of the cryogels. It was shown that modifying the PHEG-MA cryogels within the range of RGDS concentrations examined here had a positive effect on the proliferation of hDPSCs. Immunofluorescence staining for collagen type 2 and aggrecan proved that there was differentiation of hDPSCs into chondrocytes.

Keywords

cryogel, polyamino acid

Key words in English

cryogel, polyamino acid

Authors

SEDLAČÍK, T.; ACAR, O.; STUDENOVSKÁ, H.; KOTELNIKOV, I.; KUČKA, J.; KONEČNÁ, Z.; ZIKMUND, T.; KAISER, J.; KOSE, G.; RYPÁČEK, F.

RIV year

2019

Released

30.10.2017

ISBN

1744-683X

Periodical

Soft Matter

Number

14

State

United Kingdom of Great Britain and Northern Ireland

Pages from

228

Pages to

238

Pages count

11

BibTex

@article{BUT146251,
  author="Tomáš {Sedlačík} and O.K. {Acar} and Hana {Studenovská} and Ilyia {Kotelnikov} and Jan {Kučka} and Zuzana {Konečná} and Tomáš {Zikmund} and Jozef {Kaiser} and G.T. {Kose} and František {Rypáček}",
  title="Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(a-amino acids)",
  journal="Soft Matter",
  year="2017",
  number="14",
  pages="228--238",
  doi="10.1039/c7sm02074k",
  issn="1744-683X"
}