Publication result detail

ISOLATION AND DETERMINATION OF LACTOFERRINE FROM HUMAN SALIVA USING PARAMAGNETIC PARTICLES

SKALIČKOVÁ, S.; ZÍTKA, O.; KŘÍŽKOVÁ, S.; VLKOVÁ, M.; KIZEK, R.

Original Title

ISOLATION AND DETERMINATION OF LACTOFERRINE FROM HUMAN SALIVA USING PARAMAGNETIC PARTICLES

English Title

ISOLATION AND DETERMINATION OF LACTOFERRINE FROM HUMAN SALIVA USING PARAMAGNETIC PARTICLES

Type

Paper in proceedings (conference paper)

Original Abstract

Saliva is a watery substance produced in the mouths of human and most animals. The important compound of saliva is lactoferrin. It is involved in binding and transport of metal ions such as Fe2+, Zn2+, Mn2+ and Cu2+. Because of these functions it has an antibacterial, anticancerogenic and anti-inflammatory activity. The aim of this study was to design a method based on isolation of lactoferrin from human saliva using paramagnetic particles to prepare bead-based complex with lactoferrin. This complex was subsequently determined by SFIA. Our results were correlated with ELISA using spectrophotometric detection. The limit of detection for lactoferin was 0.4 mu g.ml(-1). This method has the potential as a biosensor analysis.

English abstract

Saliva is a watery substance produced in the mouths of human and most animals. The important compound of saliva is lactoferrin. It is involved in binding and transport of metal ions such as Fe2+, Zn2+, Mn2+ and Cu2+. Because of these functions it has an antibacterial, anticancerogenic and anti-inflammatory activity. The aim of this study was to design a method based on isolation of lactoferrin from human saliva using paramagnetic particles to prepare bead-based complex with lactoferrin. This complex was subsequently determined by SFIA. Our results were correlated with ELISA using spectrophotometric detection. The limit of detection for lactoferin was 0.4 mu g.ml(-1). This method has the potential as a biosensor analysis.

Keywords

lactoferrin; ELISA; SFIA; biosensor

Key words in English

lactoferrin; ELISA; SFIA; biosensor

Authors

SKALIČKOVÁ, S.; ZÍTKA, O.; KŘÍŽKOVÁ, S.; VLKOVÁ, M.; KIZEK, R.

RIV year

2018

Released

22.11.2012

ISBN

978-80-7375-836-3

Book

MENDELNET 2012

Pages from

1250

Pages to

1257

Pages count

8

BibTex

@inproceedings{BUT145755,
  author="Sylvie {Skaličková} and Ondřej {Zítka} and Soňa {Křížková} and Marcela {Vlková} and René {Kizek}",
  title="ISOLATION AND DETERMINATION OF LACTOFERRINE FROM HUMAN SALIVA USING PARAMAGNETIC PARTICLES",
  booktitle="MENDELNET 2012",
  year="2012",
  pages="1250--1257",
  isbn="978-80-7375-836-3"
}