Publication result detail

Identification of soy proteins in meat products by HPLC.

VRÁNOVÁ, D.

Original Title

Identification of soy proteins in meat products by HPLC.

English Title

Identification of soy proteins in meat products by HPLC.

Type

Paper in proceedings (conference paper)

Original Abstract

A fast, specific and sensitive method for determination of soy proteins in processed meat product has been developed. The method is based on RP-HPLC analysis of phytoestrogens daidzein and genistein using bisphenol A as an internal standard. This paper describes the development and optimization of efficient extraction procedures (n-hexane / 80 %EtOH), concentration of isoflavones by solid/phase extraction (Spe-ed Scan ABN cartridges) and conditions for gradient RP-HPLC analysis. The presence of soy proteins in different types of sausages was determined by this method. As little as 0,1% of soy protein in heated meat products could be detected and the quantitative determination of soy proteins is possible in the range 0.1- 10 %.

English abstract

A fast, specific and sensitive method for determination of soy proteins in processed meat product has been developed. The method is based on RP-HPLC analysis of phytoestrogens daidzein and genistein using bisphenol A as an internal standard. This paper describes the development and optimization of efficient extraction procedures (n-hexane / 80 %EtOH), concentration of isoflavones by solid/phase extraction (Spe-ed Scan ABN cartridges) and conditions for gradient RP-HPLC analysis. The presence of soy proteins in different types of sausages was determined by this method. As little as 0,1% of soy protein in heated meat products could be detected and the quantitative determination of soy proteins is possible in the range 0.1- 10 %.

Key words in English

meat, soy proteins, hplc

Authors

VRÁNOVÁ, D.

Released

25.05.2005

Publisher

masarykova universita

Location

Brno

ISBN

80-210-3695-8

Book

sborník abstrakt

Pages from

37

Pages to

37

Pages count

1

BibTex

@inproceedings{BUT14481,
  author="Dana {Vránová}",
  title="Identification of soy proteins in meat products by HPLC.",
  booktitle="sborník abstrakt",
  year="2005",
  number="1",
  pages="37--37",
  publisher="masarykova universita",
  address="Brno",
  isbn="80-210-3695-8"
}