Biotechnological conversion of spent coffee grounds into lactic acid

Biotechnological conversion of spent coffee grounds into lactic acid

WoS Article

This work investigates the potential bioconversion of spent coffee grounds (SCG) into lactic acid (LA). SCG were hydrolysed by a combination of dilute acid treatment and subsequent application of cellulase. The SCG hydrolysate contained a considerable amount of reducing sugars (902003gl(-1), glucose; 2649 +/- 010gl(-1) galactose and 281 +/- 007gl(-1) arabinose) and it was used as a substrate for culturing several lactic acid bacteria (LAB) and LA-producing Bacillus coagulans. Among the screened micro-organisms, Lactobacillus rhamnosus CCM 1825 was identified as the most promising producer of LA on a SCG hydrolysate. Despite the inhibitory effect exerted by furfural and phenolic compounds in the medium, reasonably high LA concentrations (2569 +/- 145gl(-1)) and yields (98%) were gained. Therefore, it could be demonstrated that SCG is a promising raw material for the production of LA and could serve as a feedstock for the sustainable large-scale production of LA. Significance and Impact of the StudySpent coffee grounds (SCG) represent solid waste generated in millions of tonnes by coffee-processing industries. Their disposal represents a serious environmental problem; however, SCG could be valorized within a biorefinery concept yielding various valuable products. Herein, we suggest that SCG can be used as a complex carbon source for the lactic acid production.

This work investigates the potential bioconversion of spent coffee grounds (SCG) into lactic acid (LA). SCG were hydrolysed by a combination of dilute acid treatment and subsequent application of cellulase. The SCG hydrolysate contained a considerable amount of reducing sugars (902003gl(-1), glucose; 2649 +/- 010gl(-1) galactose and 281 +/- 007gl(-1) arabinose) and it was used as a substrate for culturing several lactic acid bacteria (LAB) and LA-producing Bacillus coagulans. Among the screened micro-organisms, Lactobacillus rhamnosus CCM 1825 was identified as the most promising producer of LA on a SCG hydrolysate. Despite the inhibitory effect exerted by furfural and phenolic compounds in the medium, reasonably high LA concentrations (2569 +/- 145gl(-1)) and yields (98%) were gained. Therefore, it could be demonstrated that SCG is a promising raw material for the production of LA and could serve as a feedstock for the sustainable large-scale production of LA. Significance and Impact of the StudySpent coffee grounds (SCG) represent solid waste generated in millions of tonnes by coffee-processing industries. Their disposal represents a serious environmental problem; however, SCG could be valorized within a biorefinery concept yielding various valuable products. Herein, we suggest that SCG can be used as a complex carbon source for the lactic acid production.

Lactic acid bacteria, fermentation, Lactobacillus, waste, biotechnology, spent coffee grounds, lactic acid

Lactic acid bacteria, fermentation, Lactobacillus, waste, biotechnology, spent coffee grounds, lactic acid

HUDEČKOVÁ, H.; NEUREITER, M.; OBRUČA, S.; FRÜHAUF, S.; MÁROVÁ, I.

2019

13.01.2018

0266-8254

LETTERS IN APPLIED MICROBIOLOGY

66

4

United Kingdom of Great Britain and Northern Ireland

306

312

7

https://onlinelibrary.wiley.com/doi/epdf/10.1111/lam.12849

http://hdl.handle.net/