Publication detail

FireProt: web server for automated design of thermostable proteins

MUSIL, M. ŠTOURAČ, J. BENDL, J. BREZOVSKÝ, J. PROKOP, Z. ZENDULKA, J. MARTÍNEK, T. BEDNÁŘ, D. DAMBORSKÝ, J.

Original Title

FireProt: web server for automated design of thermostable proteins

Type

journal article in Web of Science

Language

English

Original Abstract

There is a continuous interest in increasing proteins stability to enhance their usability in numerous biomedical and biotechnological applications. A number of in silico tools for the prediction of the effect of mutations on protein stability have been developed recently. However, only single-point mutations with a small effect on protein stability are typically predicted with the existing tools and have to be followed by laborious protein expression, purification, and characterization. Here, we present FireProt, a web server for the automated design of multiplepoint thermostable mutant proteins that combines structural and evolutionary information in its calculation core. FireProt utilizes sixteen tools and three protein engineering strategies for making reliable protein designs. The server is complemented with interactive, easy-to-use interface that allows users to directly analyze and optionally modify designed thermostable mutants. FireProt is freely available at http://loschmidt.chemi.muni.cz/fireprot.

Keywords

mutations, protein engineering, mutant proteins, protein stability, thermostable proteins

Authors

MUSIL, M.; ŠTOURAČ, J.; BENDL, J.; BREZOVSKÝ, J.; PROKOP, Z.; ZENDULKA, J.; MARTÍNEK, T.; BEDNÁŘ, D.; DAMBORSKÝ, J.

Released

26. 4. 2017

ISBN

1362-4962

Periodical

Nucleic Acids Research

Year of study

45

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

393

Pages to

399

Pages count

7

URL

https://doi.org/10.1093/nar/gkx285

BibTex

@article{BUT144434,
  author="Miloš {Musil} and Jan {Štourač} and Jaroslav {Bendl} and Jan {Brezovský} and Zbyněk {Prokop} and Jaroslav {Zendulka} and Tomáš {Martínek} and David {Bednář} and Jiří {Damborský}",
  title="FireProt: web server for automated design of thermostable proteins",
  journal="Nucleic Acids Research",
  year="2017",
  volume="45",
  number="1",
  pages="393--399",
  doi="10.1093/nar/gkx285",
  issn="1362-4962",
  url="https://doi.org/10.1093/nar/gkx285"
}