Publication result detail

Production and characterization of extracellular polysaccharide of extremely halophilic Archaea Haloferax mediterranei

CHYTILOVÁ, A.; DRÁBKOVÁ, K.; STREČANSKÁ, P.; ENEV, V.; KUČERA, D.; BENEŠOVÁ, P.; OBRUČA, S.

Original Title

Production and characterization of extracellular polysaccharide of extremely halophilic Archaea Haloferax mediterranei

English Title

Production and characterization of extracellular polysaccharide of extremely halophilic Archaea Haloferax mediterranei

Type

Abstract

Original Abstract

Haloferax mediterranei is extremely halophilic archeon capable of production of two very interesting biopolymers. This microorganism intracellularly accumulates polyhydroxyalkanoates (PHA) while simultaneously excreting polysaccharide. In this work we focused on production and characterization of its extracellular polysaccharide. To decrease cost of the cultivation media we substituted expensive yeast extract by alkaline hydrolyzed waste chicken feather which positively influenced polysaccharide yields.Moreover, isolated polysaccharide was capable to provide very high protective activity for model prokaryotic (Cupriavidus necator) and eukaryotic (Saccharomyces cerevisiae) microorganisms against various stress factors such as freezing, ethanol, high temperature, osmotic pressure, oxidative pressure induced by H2O2 or SDS which imply its interesting biological properties and potential applications in biotechnology or health care.

English abstract

Haloferax mediterranei is extremely halophilic archeon capable of production of two very interesting biopolymers. This microorganism intracellularly accumulates polyhydroxyalkanoates (PHA) while simultaneously excreting polysaccharide. In this work we focused on production and characterization of its extracellular polysaccharide. To decrease cost of the cultivation media we substituted expensive yeast extract by alkaline hydrolyzed waste chicken feather which positively influenced polysaccharide yields.Moreover, isolated polysaccharide was capable to provide very high protective activity for model prokaryotic (Cupriavidus necator) and eukaryotic (Saccharomyces cerevisiae) microorganisms against various stress factors such as freezing, ethanol, high temperature, osmotic pressure, oxidative pressure induced by H2O2 or SDS which imply its interesting biological properties and potential applications in biotechnology or health care.

Keywords

Haloferax mediterranei, Cupriavidus necator, extracellular polysaccharide, Raman spectroscopy, FTIR and elemental analysis

Key words in English

Haloferax mediterranei, Cupriavidus necator, extracellular polysaccharide, Raman spectroscopy, FTIR and elemental analysis

Authors

CHYTILOVÁ, A.; DRÁBKOVÁ, K.; STREČANSKÁ, P.; ENEV, V.; KUČERA, D.; BENEŠOVÁ, P.; OBRUČA, S.

RIV year

2018

Released

11.09.2017

Location

Krakow

Book

6th Central European Congress of Life Sciences Eurobiotech

Pages from

156

Pages to

157

Pages count

1

BibTex

@misc{BUT142931,
  author="Aneta {Chytilová} and Kateřina {Drábková} and Paulína {Strečanská} and Vojtěch {Enev} and Dan {Kučera} and Pavla {Benešová} and Stanislav {Obruča}",
  title="Production and characterization of extracellular polysaccharide of extremely halophilic Archaea Haloferax mediterranei",
  booktitle="6th Central European Congress of Life Sciences Eurobiotech",
  year="2017",
  pages="156--157",
  address="Krakow",
  note="Abstract"
}