Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media

Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media

Paper in proceedings (conference paper)

In this work we present the coherence controlled holographic microscopy (CCHM)(1) and its ability to image the living cells in turbid media(2). The CCHM method and its advantages are introduced. A 'coherence gate effect'(3), that enables imaging in turbid media, occurs owing to the low coherence illumination in our setup. The coherence gate effect is briefly theoretically explained and comparison of images with different illumination sources is shown. After that, the possibility of imaging in turbid media is applied to investigation of cell reactions to cytopathic turbid emulsions. In our experiments we used human cancer cells treated by biologically active phospholipids (BAPs). Cellular events leading to cell death, that would otherwise remain hidden in turbid media, are clearly observable and according to them cell fate can be deduced.

In this work we present the coherence controlled holographic microscopy (CCHM)(1) and its ability to image the living cells in turbid media(2). The CCHM method and its advantages are introduced. A 'coherence gate effect'(3), that enables imaging in turbid media, occurs owing to the low coherence illumination in our setup. The coherence gate effect is briefly theoretically explained and comparison of images with different illumination sources is shown. After that, the possibility of imaging in turbid media is applied to investigation of cell reactions to cytopathic turbid emulsions. In our experiments we used human cancer cells treated by biologically active phospholipids (BAPs). Cellular events leading to cell death, that would otherwise remain hidden in turbid media, are clearly observable and according to them cell fate can be deduced.

holographic microscopy; quantitative phase imaging; coherence gate effect; live cells; turbid media; low coherent interferometry; cancer treatment

holographic microscopy; quantitative phase imaging; coherence gate effect; live cells; turbid media; low coherent interferometry; cancer treatment

LOŠŤÁK, M.; ČOLLÁKOVÁ, J.; SLABÝ, T.; KŘÍŽOVÁ, A.; VESELÝ, P.; CHMELÍK, R.

2018

09.03.2016

978-1-62841-952-8

QUANTITATIVE PHASE IMAGING II

0277-786X

Proceedings of SPIE

9718

United States of America

97181Y-1

97181Y-5

5

https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9718/1/Coherence-controlled-holographic-microscopy-for-live-cell-quantitative-phase-imaging/10.1117/12.2209322.short