Publication result detail

Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions

MONTUFAR JIMENEZ, E.; VOJTOVÁ, L.; ČELKO, L.; GINEBRA, M. P.

Original Title

Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions

English Title

Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions

Type

Chapter in a book

Original Abstract

The present method describes the procedure to fabricate calcium phosphate foams with suitable open porosity, pore size, and composition to perform three-dimensional (3D) cell cultures with the objective to simulate the bone tissue microenvironment in vitro. Foams with two compositions but equivalent porosity can be fabricated. On the one hand, hydroxyapatite foams obtained by hydrolysis at 37 °C, with microstructure that mimics the small crystal size of the mineral component of bones, and on the other hand, beta tricalcium phosphate foams with polygonal grains obtained by sintering at 1100 °C. In the first part of the chapter the calcium phosphate foams are briefly described. Afterwards, the foaming process is described in detail, including alternatives to overcome processing problems than can arise. Finally, insights are provided on how to perform 3D cell cultures using the calcium phosphate foams as substrates.

English abstract

The present method describes the procedure to fabricate calcium phosphate foams with suitable open porosity, pore size, and composition to perform three-dimensional (3D) cell cultures with the objective to simulate the bone tissue microenvironment in vitro. Foams with two compositions but equivalent porosity can be fabricated. On the one hand, hydroxyapatite foams obtained by hydrolysis at 37 °C, with microstructure that mimics the small crystal size of the mineral component of bones, and on the other hand, beta tricalcium phosphate foams with polygonal grains obtained by sintering at 1100 °C. In the first part of the chapter the calcium phosphate foams are briefly described. Afterwards, the foaming process is described in detail, including alternatives to overcome processing problems than can arise. Finally, insights are provided on how to perform 3D cell cultures using the calcium phosphate foams as substrates.

Keywords

Calcium phosphate foam; Hydroxyapatite; Tricalcium phosphate; Bone model; Cell culture; Three-dimensional; Osteoblast; Tissue engineering; Scaffold

Key words in English

Calcium phosphate foam; Hydroxyapatite; Tricalcium phosphate; Bone model; Cell culture; Three-dimensional; Osteoblast; Tissue engineering; Scaffold

Authors

MONTUFAR JIMENEZ, E.; VOJTOVÁ, L.; ČELKO, L.; GINEBRA, M. P.

RIV year

2019

Released

21.06.2017

Publisher

Springer

ISBN

978-1-4939-7019-3

Book

Methods in Molecular Biology

Edition

1612

Pages from

79

Pages to

94

Pages count

16

URL

https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_6

BibTex

@inbook{BUT138650,
  author="MONTUFAR JIMENEZ, E. and VOJTOVÁ, L. and ČELKO, L. and GINEBRA, M. P.",
  title="Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions",
  booktitle="Methods in Molecular Biology",
  year="2017",
  publisher="Springer",
  series="1612",
  pages="79--94",
  doi="10.1007/978-1-4939-7021-6\{_}6",
  isbn="978-1-4939-7019-3",
  url="https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_6"
}