In vivo fluorescence visualization of quantum dot nanoparticles in plants

In vivo fluorescence visualization of quantum dot nanoparticles in plants

Paper in proceedings (conference paper)

Visualization of nanoparticles can be exceedingly useful in tracking of targeted drug delivery systems. For this purpose, highly luminescent quantum dot nanoparticles may represent a suitable option due to their superior photophysical properties and versatile surface chemistry. This study was mainly focused on application of CdTe quantum dot nanoparticles for fluorescence imaging of their transport in plants. In vivo experiments were carried using leaves of sunflower plant (Helianthus annuus). Leaves, soaking water solution of CdTe-PVP and CdTe/ZnS quantum dots, were monitored (ʎem 535 nm and 600 nm respectively) for 8 hours at time intervals of 60 minutes using In Vivo Xtreme Imaging System (Bruker, MA, USA). Autofluorescence of biomolecules present in plants, including chlorophyll, carotene and xanthophylls, represents a crucial problem in fluorescence imaging of plants. However, by using adequate excitation and emission filters during fluorescence images acquisition, this phenomenon can be effectively suppressed. Moreover, multispectral imaging and spectral modelling can be performed in order to distinguish fluorescence of quantum dots. In this study, a comparison of two different modifications of CdTe quantum dots is provided together with recommendations on setting of appropriate excitation and emission range for image acquisition.

Visualization of nanoparticles can be exceedingly useful in tracking of targeted drug delivery systems. For this purpose, highly luminescent quantum dot nanoparticles may represent a suitable option due to their superior photophysical properties and versatile surface chemistry. This study was mainly focused on application of CdTe quantum dot nanoparticles for fluorescence imaging of their transport in plants. In vivo experiments were carried using leaves of sunflower plant (Helianthus annuus). Leaves, soaking water solution of CdTe-PVP and CdTe/ZnS quantum dots, were monitored (ʎem 535 nm and 600 nm respectively) for 8 hours at time intervals of 60 minutes using In Vivo Xtreme Imaging System (Bruker, MA, USA). Autofluorescence of biomolecules present in plants, including chlorophyll, carotene and xanthophylls, represents a crucial problem in fluorescence imaging of plants. However, by using adequate excitation and emission filters during fluorescence images acquisition, this phenomenon can be effectively suppressed. Moreover, multispectral imaging and spectral modelling can be performed in order to distinguish fluorescence of quantum dots. In this study, a comparison of two different modifications of CdTe quantum dots is provided together with recommendations on setting of appropriate excitation and emission range for image acquisition.

CdTe quantum dots; fluorescence in vivo imaging; Helianthus annuus; nanoparticles transport monitoring

CdTe quantum dots; fluorescence in vivo imaging; Helianthus annuus; nanoparticles transport monitoring

VANĚČKOVÁ, T.; ŠTUŘÍKOVÁ, H.; MILOSAVLJEVIĆ, V.; KOPEL, P.; KRYŠTOFOVÁ, O.; VACULOVIČOVÁ, M.; ADAM, V.

2018

09.11.2016

Mendel University in Brno

Brno, Czech Republic

978-80-7509-443-8

MendelNet2016

1026

1030

5

Vanecková