Cyclopropylamine plasma polymers for increased cell adhesion and growth

Cyclopropylamine plasma polymers for increased cell adhesion and growth

WoS Article

Plasma polymers with NHx groups were deposited from cyclopropylamine in low pressure radio frequency (RF) discharges. The amount of NHx decreased with increasing average power Pav, whereas the layer dissolution in water decreased and high Pav led to a slight film swelling. The C2C12 cells demonstrated very high adhesion to the layers regardless of the deposition conditions. Although the surface chemistry of amine-rich layers should enhance the cell proliferation, the WST-1 assay, and immunocytochemistry revealed lower cell proliferation and viability on the layers exhibiting above 18% of relative thickness loss in water. Promising results were obtained on the layers with better water stability and bearing 7–10% of NHx.

Plasma polymers with NHx groups were deposited from cyclopropylamine in low pressure radio frequency (RF) discharges. The amount of NHx decreased with increasing average power Pav, whereas the layer dissolution in water decreased and high Pav led to a slight film swelling. The C2C12 cells demonstrated very high adhesion to the layers regardless of the deposition conditions. Although the surface chemistry of amine-rich layers should enhance the cell proliferation, the WST-1 assay, and immunocytochemistry revealed lower cell proliferation and viability on the layers exhibiting above 18% of relative thickness loss in water. Promising results were obtained on the layers with better water stability and bearing 7–10% of NHx.

amine coatings; cell adhesion; cyclopropylamine; ESCA/XPS;, pulsed discharges

amine coatings; cell adhesion; cyclopropylamine; ESCA/XPS;, pulsed discharges

MANAKHOV, A.; LANDOVÁ, M.; MEDALOVÁ, J.; MICHLÍČEK, M.; POLČÁK, J.; NEČAS, D.; ZAJÍČKOVÁ, L.

2018

18.07.2017

1612-8869

Plasma Processes and Polymers

14

7

Federal Republic of Germany

e1600123-1

e1600123-1

12