Detail publikačního výsledku

Reclassification of non-type strain Clostridium pasteurianum NRRL B-598 as Clostridium beijerinckii NRRL B-598

SEDLÁŘ, K.; KOLEK, J.; PROVAZNÍK, I.; PATÁKOVÁ, P.

Original Title

Reclassification of non-type strain Clostridium pasteurianum NRRL B-598 as Clostridium beijerinckii NRRL B-598

English Title

Reclassification of non-type strain Clostridium pasteurianum NRRL B-598 as Clostridium beijerinckii NRRL B-598

Type

WoS Article

Original Abstract

The complete genome sequence of non-type strain Clostridium pasteurianum NRRL B-598 was introduced last year; it is an oxygen tolerant, spore-forming, mesophilic heterofermentative bacterium with high hydrogen production and acetone-butanol fermentation ability. The basic genome statistics have shown its similarity to C. beijerinckii rather than the C. pasteurianum species. Here, we present a comparative analysis of the strain with several other complete clostridial genome sequences. Besides a 16S rRNA gene sequence comparison, digital DNA–DNA hybridization (dDDH) and phylogenomic analysis confirmed an inaccuracy of the taxonomic status of strain Clostridium pasteurianum NRRL B-598. Therefore, we suggest its reclassification to be Clostridium beijerinckii NRRL B-598. This is a specific strain and is not identical to other C. beijerinckii strains. This misclassification explains its unexpected behavior, different from other C. pasteurianum strains; it also permits better understanding of the bacterium for a future genetic manipulation that might increase its biofuel production potential.

English abstract

The complete genome sequence of non-type strain Clostridium pasteurianum NRRL B-598 was introduced last year; it is an oxygen tolerant, spore-forming, mesophilic heterofermentative bacterium with high hydrogen production and acetone-butanol fermentation ability. The basic genome statistics have shown its similarity to C. beijerinckii rather than the C. pasteurianum species. Here, we present a comparative analysis of the strain with several other complete clostridial genome sequences. Besides a 16S rRNA gene sequence comparison, digital DNA–DNA hybridization (dDDH) and phylogenomic analysis confirmed an inaccuracy of the taxonomic status of strain Clostridium pasteurianum NRRL B-598. Therefore, we suggest its reclassification to be Clostridium beijerinckii NRRL B-598. This is a specific strain and is not identical to other C. beijerinckii strains. This misclassification explains its unexpected behavior, different from other C. pasteurianum strains; it also permits better understanding of the bacterium for a future genetic manipulation that might increase its biofuel production potential.

Keywords

Clostridium pasteurianum; Clostridium beijerinckii; Reclassification; Phylogenomic analysis

Key words in English

Clostridium pasteurianum; Clostridium beijerinckii; Reclassification; Phylogenomic analysis

Authors

SEDLÁŘ, K.; KOLEK, J.; PROVAZNÍK, I.; PATÁKOVÁ, P.

RIV year

2018

Released

19.01.2017

Publisher

Elsevier

ISBN

0168-1656

Periodical

JOURNAL OF BIOTECHNOLOGY

Volume

244

Number

1

State

Kingdom of the Netherlands

Pages from

1

Pages to

3

Pages count

3

URL

http://www.sciencedirect.com/science/article/pii/S0168165617300135

Full text in the Digital Library

http://hdl.handle.net/

BibTex

@article{BUT132211,
  author="Karel {Sedlář} and Jan {Kolek} and Valentýna {Provazník} and Petra {Patáková}",
  title="Reclassification of non-type strain Clostridium pasteurianum NRRL B-598 as Clostridium beijerinckii NRRL B-598",
  journal="JOURNAL OF BIOTECHNOLOGY",
  year="2017",
  volume="244",
  number="1",
  pages="1--3",
  doi="10.1016/j.jbiotec.2017.01.003",
  issn="0168-1656",
  url="http://www.sciencedirect.com/science/article/pii/S0168165617300135"
}