Applied result detail

FireProt web

MUSIL, M.; ŠTOURAČ, J.; BENDL, J.; BREZOVSKÝ, J.; MARTÍNEK, T.; ZENDULKA, J.; PROKOP, Z.; BEDNÁŘ, D.; DAMBORSKÝ, J.

Original Title

FireProt web

English Title

FireProt web

Type

Software

Abstract

FireProt is a web server for an automated design of thermostable mutants. The design of thermostable mutants is based on integration of structural and evolutionary infromation obtained from several bioinfromatics databases and computational tools.
FireProt strategy combines energy and evolution based approaches together with several filters that accelerate the calculation by omitting potentially deleterious mutations. Within its workflow, FireProt integrates 16 computational tools, utilizing both sequence and structural information in the process. FIreProt web server provides users with a one-stop-shop solution for the design of thermostable proteins, constructed by three distinct strategies. Futhermore, the server allows users to include user-defined mutations into the constructed thermostable protein and generate corresponding sequences in the FASTA format.

Abstract in English

FireProt is a web server for an automated design of thermostable mutants. The design of thermostable mutants is based on integration of structural and evolutionary infromation obtained from several bioinfromatics databases and computational tools.
FireProt strategy combines energy and evolution based approaches together with several filters that accelerate the calculation by omitting potentially deleterious mutations. Within its workflow, FireProt integrates 16 computational tools, utilizing both sequence and structural information in the process. FIreProt web server provides users with a one-stop-shop solution for the design of thermostable proteins, constructed by three distinct strategies. Futhermore, the server allows users to include user-defined mutations into the constructed thermostable protein and generate corresponding sequences in the FASTA format.

Keywords

protein engineering, thermostable proteins, multiple-point mutants, protein stability

Key words in English

protein engineering, thermostable proteins, multiple-point mutants, protein stability

Location

Nástroj je dostupný na internetové adrese: http://loschmidt.chemi.muni.cz/fireprot

Licence fee

Use of the result by another entity is possible without acquiring a license in some cases

www

https://www.fit.vut.cz/research/product/508/