Publication result detail

Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application

STANISAVLJEVIC, M.; KŘÍŽKOVÁ, S.; VACULOVIČOVÁ, M.; KIZEK, R.; ADAM, V.

Original Title

Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application

English Title

Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application

Type

WoS Article

Original Abstract

Fluorescence resonance energy transfer (FRET)-based nanosensors with quantum dots (QDs) as donors and organic dyes as acceptors have long been of interest for the detection of biomolecules such as nucleic acids, but their low FRET efficiency in bulk solution has prevented the sensitive detection of nucleic acids due to the large size of the QDs and the long length of nucleic acids. Here we describe a novel approach to improve the detection sensitivity of QD-based nanosensors using single-molecule detection in a capillary flow. In comparison with bulk measurement, single-molecule detection in a capillary flow possesses the unique advantages of improved FRET efficiency, high sensitivity, prevention of photobleaching, and low sample consumption. Greater FRET efficiency was obtained due to the deformation of DNA in the capillary stream. This technique can be easily extended to sensitive bimolecular analysis in microfluidic chips, and it may also offer a promising approach to study the deformation of small nucleic acids in fluid flow.

English abstract

Fluorescence resonance energy transfer (FRET)-based nanosensors with quantum dots (QDs) as donors and organic dyes as acceptors have long been of interest for the detection of biomolecules such as nucleic acids, but their low FRET efficiency in bulk solution has prevented the sensitive detection of nucleic acids due to the large size of the QDs and the long length of nucleic acids. Here we describe a novel approach to improve the detection sensitivity of QD-based nanosensors using single-molecule detection in a capillary flow. In comparison with bulk measurement, single-molecule detection in a capillary flow possesses the unique advantages of improved FRET efficiency, high sensitivity, prevention of photobleaching, and low sample consumption. Greater FRET efficiency was obtained due to the deformation of DNA in the capillary stream. This technique can be easily extended to sensitive bimolecular analysis in microfluidic chips, and it may also offer a promising approach to study the deformation of small nucleic acids in fluid flow.

Keywords

Quantum dots, Fluorescence resonanceenergytransfer, Sensor, Nucleicacid,Enzyme

Key words in English

Quantum dots, Fluorescence resonanceenergytransfer, Sensor, Nucleicacid,Enzyme

Authors

STANISAVLJEVIC, M.; KŘÍŽKOVÁ, S.; VACULOVIČOVÁ, M.; KIZEK, R.; ADAM, V.

RIV year

2016

Released

15.12.2015

ISBN

0956-5663

Periodical

BIOSENSORS & BIOELECTRONICS

Volume

74

Number

1

State

Kingdom of the Netherlands

Pages from

562

Pages to

574

Pages count

13

BibTex

@article{BUT121157,
  author="Maja {Stanisavljevic} and Soňa {Křížková} and Markéta {Vaculovičová} and René {Kizek} and Vojtěch {Adam}",
  title="Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application",
  journal="BIOSENSORS & BIOELECTRONICS",
  year="2015",
  volume="74",
  number="1",
  pages="562--574",
  doi="10.1016/j.bios.2015.06.076",
  issn="0956-5663"
}