Novel Oxycellulose/Gelatin Nanofibers for Medical Applications

Novel Oxycellulose/Gelatin Nanofibers for Medical Applications

Abstract

Novel hydrolytically stable gelatin nanofibers were modified with sodium or calcium salts of oxidized cellulose (oxycellulose) to enhance nanofibers’ stability and bactericidal efficiency. The unique inhibitory effect of the electrospun gelatin/ oxycellulose nanofibers against pathogenic bacteria was examined by luminometric method using modified bioluminescence of E. coli strain. Non-woven nanofiber fabric has been tested for its biocompatibility using human lung adenocarcinoma cells line NCI-H441. Cells cultured on nanofibrous materials firmly adhered to their surface without the changing in shape, as determined by scanning electron microscopy, and did not show any signs of dying as revealed by live/death staining via fluorescence microscope. This newly developed nanofibrous material seeded with NCI-H441 cell line could be therefore used as a platform for lung disease modeling and anti-cancer drug testing. Moreover, novel bactericidal and hemostatic nanofibers, non-toxic to eukaryotic cells represent a promising option for application in surgery and regenerative medicine, particularly in soft tissue engineering.

Novel hydrolytically stable gelatin nanofibers were modified with sodium or calcium salts of oxidized cellulose (oxycellulose) to enhance nanofibers’ stability and bactericidal efficiency. The unique inhibitory effect of the electrospun gelatin/ oxycellulose nanofibers against pathogenic bacteria was examined by luminometric method using modified bioluminescence of E. coli strain. Non-woven nanofiber fabric has been tested for its biocompatibility using human lung adenocarcinoma cells line NCI-H441. Cells cultured on nanofibrous materials firmly adhered to their surface without the changing in shape, as determined by scanning electron microscopy, and did not show any signs of dying as revealed by live/death staining via fluorescence microscope. This newly developed nanofibrous material seeded with NCI-H441 cell line could be therefore used as a platform for lung disease modeling and anti-cancer drug testing. Moreover, novel bactericidal and hemostatic nanofibers, non-toxic to eukaryotic cells represent a promising option for application in surgery and regenerative medicine, particularly in soft tissue engineering.

electrospinning, biocompatibility, hydrolytical stability, crosslinking, medicine

electrospinning, biocompatibility, hydrolytical stability, crosslinking, medicine

ŠVACHOVÁ, V.; VOJTOVÁ, L.; PAVLIŇÁK, D.; FOHLEROVÁ, Z.

2016

03.12.2015

Vysoké učení technické v Brně

Brno

978-80-214-5290-6

Sborník příspěvků; Studentská konference Chemie je život

první

367

367

1

http://www.fch.vutbr.cz/media/sok/sbornik_prispevku_2015.pdf