Publication result detail

Optimization of derivatization of acidic drugs for analysis by GC-MS and its application for determination of drug residues in wastewater

SÝKORA, R.; VÁVROVÁ, M.; MRAVCOVÁ, L.; LACINA, P.

Original Title

Optimization of derivatization of acidic drugs for analysis by GC-MS and its application for determination of drug residues in wastewater

English Title

Optimization of derivatization of acidic drugs for analysis by GC-MS and its application for determination of drug residues in wastewater

Type

WoS Article

Original Abstract

This article is focused on optimization of derivatization of acidic drugs from the group of non-steroidal anti-inflammatory drugs (NSAID): salicylic acid, ibuprofen, naproxen, ketoprofen and diclofenac. Derivatization is a necessary step for analysis of pharmaceuticals by GC. MSTFA (N-methylN-(trimethylsilyl)trifluoroacetamide) and BSTFA (N,Obis(trimethylsilyl)trifluoroacetamide) were used as a derivatization reagents and tested under different conditions: volume, temperature and time of derivatization. Results of optimization were compared and optimized conditions were successfully applied for analysis of real samples of wastewater. Wastewater samples were collected from influent and effluent of wastewater treatment plant (WWTP). The whole analytical procedure involved solid phase extraction (SPE), optimized derivatization and analysis by GC-TOF MS. Limits of detection (LOD) ranged between 8.9 - 71.6 ng.L-1 and limits of quantification (LOQ) ranged between 29.5 – 238.7 ng.L-1 depending on the specific compound. Concentration of selected drugs in real samples of wastewater varied from 0.1 to 30 g.L-1 in influent and from 0.05 to 2 g.L-1 in effluent, depending on the compound.

English abstract

This article is focused on optimization of derivatization of acidic drugs from the group of non-steroidal anti-inflammatory drugs (NSAID): salicylic acid, ibuprofen, naproxen, ketoprofen and diclofenac. Derivatization is a necessary step for analysis of pharmaceuticals by GC. MSTFA (N-methylN-(trimethylsilyl)trifluoroacetamide) and BSTFA (N,Obis(trimethylsilyl)trifluoroacetamide) were used as a derivatization reagents and tested under different conditions: volume, temperature and time of derivatization. Results of optimization were compared and optimized conditions were successfully applied for analysis of real samples of wastewater. Wastewater samples were collected from influent and effluent of wastewater treatment plant (WWTP). The whole analytical procedure involved solid phase extraction (SPE), optimized derivatization and analysis by GC-TOF MS. Limits of detection (LOD) ranged between 8.9 - 71.6 ng.L-1 and limits of quantification (LOQ) ranged between 29.5 – 238.7 ng.L-1 depending on the specific compound. Concentration of selected drugs in real samples of wastewater varied from 0.1 to 30 g.L-1 in influent and from 0.05 to 2 g.L-1 in effluent, depending on the compound.

Keywords

Non-steroidal anti-inflammatory drugs, derivatization, silylation, derivatization reagents, gas chromatography, wastewater

Key words in English

Non-steroidal anti-inflammatory drugs, derivatization, silylation, derivatization reagents, gas chromatography, wastewater

Authors

SÝKORA, R.; VÁVROVÁ, M.; MRAVCOVÁ, L.; LACINA, P.

RIV year

2016

Released

01.12.2015

ISBN

1018-4619

Periodical

Fresenius Environmental Bulletin

Volume

24

Number

11a

State

Federal Republic of Germany

Pages from

3813

Pages to

3821

Pages count

9

BibTex

@article{BUT118998,
  author="Richard {Sýkora} and Milada {Vávrová} and Ludmila {Mravcová} and Petr {Lacina}",
  title="Optimization of derivatization of acidic drugs for analysis by GC-MS and its application for determination of drug residues in wastewater",
  journal="Fresenius Environmental Bulletin",
  year="2015",
  volume="24",
  number="11a",
  pages="3813--3821",
  issn="1018-4619"
}