Publication result detail

Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine

KOMÍNKOVÁ, M.; HORKÝ, P.; KIZEK, R.; CERNEI, N.; TMEJOVÁ, K.; RUTTKAY-NEDECKÝ, B.; GURÁŇ, R.; POHANKA, M.; ZÍTKA, O.; ADAM, V.

Original Title

Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine

English Title

Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine

Type

WoS Article

Original Abstract

Taurine exhibits many positive effects on organism, such as induction of important protective biochemical mechanisms and antioxidant effects. In this work, we studied the effect of taurine consumption on levels of taurine, reduced glutathione (GSH) and metallothionein in liver and brain tissues of Wistar rats. The aim of the experiment was the monitoring of changes in concentration of taurine in brain and liver tissues and also changes in concentration of GSH and metallothionein. After optimization, the limit of detection of GSH in brain and liver tissue was established as 40 nmol.g-1 of protein and 90 nmol.g-1 of protein, respectively. That is sufficient for determination of the concentration alterations of GSH in studied tissues. The consumption of taurine increased content of GSH determined in the brain and liver (increased by approximately 0.8 mmol.g-1 of protein in comparison with control). The increase of concentration of taurine occurred only in the brain, by cca 50 μg.g-1 of tissue, regardless the applied concentration of taurine. The applied concentration of taurine had no effect on concentration of metallothionein in any of the examined tissue.

English abstract

Taurine exhibits many positive effects on organism, such as induction of important protective biochemical mechanisms and antioxidant effects. In this work, we studied the effect of taurine consumption on levels of taurine, reduced glutathione (GSH) and metallothionein in liver and brain tissues of Wistar rats. The aim of the experiment was the monitoring of changes in concentration of taurine in brain and liver tissues and also changes in concentration of GSH and metallothionein. After optimization, the limit of detection of GSH in brain and liver tissue was established as 40 nmol.g-1 of protein and 90 nmol.g-1 of protein, respectively. That is sufficient for determination of the concentration alterations of GSH in studied tissues. The consumption of taurine increased content of GSH determined in the brain and liver (increased by approximately 0.8 mmol.g-1 of protein in comparison with control). The increase of concentration of taurine occurred only in the brain, by cca 50 μg.g-1 of tissue, regardless the applied concentration of taurine. The applied concentration of taurine had no effect on concentration of metallothionein in any of the examined tissue.

Keywords

glutathione; taurine; metallothionein; antioxidant; amino acids; rats; HPLC

Key words in English

glutathione; taurine; metallothionein; antioxidant; amino acids; rats; HPLC

Authors

KOMÍNKOVÁ, M.; HORKÝ, P.; KIZEK, R.; CERNEI, N.; TMEJOVÁ, K.; RUTTKAY-NEDECKÝ, B.; GURÁŇ, R.; POHANKA, M.; ZÍTKA, O.; ADAM, V.

RIV year

2016

Released

30.12.2014

ISBN

1452-3981

Periodical

International Journal of Electrochemical Science

Volume

10

Number

2

State

Republic of Serbia

Pages from

1716

Pages to

1727

Pages count

12

BibTex

@article{BUT118726,
  author="Markéta {Komínková} and Pavel {Horký} and René {Kizek} and Natalia Vladimirovna {Cernei} and Kateřina {Tmejová} and Branislav {Ruttkay-Nedecký} and Roman {Guráň} and Miroslav {Pohanka} and Ondřej {Zítka} and Vojtěch {Adam}",
  title="Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine",
  journal="International Journal of Electrochemical Science",
  year="2014",
  volume="10",
  number="2",
  pages="1716--1727",
  issn="1452-3981"
}