Publication detail

A threshold of mechanical strain intensity for the direct activation of osteoblast function exists in a murine maxilla loading model

SUZUKI, N. AOKI, K. MARCIÁN, P. BORÁK, L. WAKABAYASHI, N.

Original Title

A threshold of mechanical strain intensity for the direct activation of osteoblast function exists in a murine maxilla loading model

Type

journal article in Web of Science

Language

English

Original Abstract

The response to the mechanical loading of bone tissue has been extensively investigated; however, precisely how much strain intensity is necessary to promote bone formation remains unclear. Combination studies utilizing histomorphometric and numerical analyses were performed using the established murine maxilla loading model to clarify the threshold of mechanical strain needed to accelerate bone formation activity. For 7 days, 191 kPa loading stimulation for 30 min/day was applied to C57BL/6J mice. Two regions of interest, the AWAY region (away from the loading site) and the NEAR region (near the loading site), were determined. The inflammatory score increased in the NEAR region, but not in the AWAY region. A strain intensity map obtained from microCT images was superimposed onto the images of the bone formation inhibitor, sclerostin-positive cell localization. The number of sclerostin-positive cells significantly decreased after mechanical loading of more than 150 micro strain in the AWAY region, but not in the NEAR region. The mineral apposition rate, which shows the bone formation ability of osteoblasts, was accelerated at the site of surface strain intensity, namely around 170 micro strain, but not at the site of lower surface strain intensity, which was around 80 micro strain in the AWAY region, thus suggesting the existence of a strain intensity threshold for promoting bone formation. Taken together, our data suggest that a threshold of mechanical strain intensity for the direct activation of osteoblast function and the reduction of sclerostin exists in a murine maxilla loading model in the non-inflammatory region.

Keywords

Mechanical strain intensity, Murine maxilla, Bone formation, Inflammation, Sclerostin, Numerical analysis

Authors

SUZUKI, N.; AOKI, K.; MARCIÁN, P.; BORÁK, L.; WAKABAYASHI, N.

Released

1. 10. 2016

Publisher

Springer

Location

Germany

ISBN

1617-7959

Periodical

Biomechanics and Modeling in Mechanobiology

Year of study

15

Number

5

State

Federal Republic of Germany

Pages from

1091

Pages to

1100

Pages count

10

URL

http://link.springer.com/article/10.1007/s10237-015-0746-1

BibTex

@article{BUT118326,
  author="Natsuki {Suzuki} and Kazuhiro {Aoki} and Petr {Marcián} and Libor {Borák} and Noriyuki {WAKABAYASHI}",
  title="A threshold of mechanical strain intensity for the direct activation of osteoblast function exists in a murine maxilla loading model",
  journal="Biomechanics and Modeling in Mechanobiology",
  year="2016",
  volume="15",
  number="5",
  pages="1091--1100",
  doi="10.1007/s10237-015-0746-1",
  issn="1617-7959",
  url="http://link.springer.com/article/10.1007/s10237-015-0746-1"
}