Detail publikačního výsledku

Voltage Sensitive Dye di-4-ANNEPS Prolongs Impulse Conduction Through Ventricles, but not Through AV Node in Isolated Rabbit Heart

OLEJNÍČKOVÁ, V.; RONZHINA, M.; JANOUŠEK, O.; KOLÁŘOVÁ, J.; FIALOVÁ, K.; PROVAZNÍK, I.; NOVÁKOVÁ, M.

Original Title

Voltage Sensitive Dye di-4-ANNEPS Prolongs Impulse Conduction Through Ventricles, but not Through AV Node in Isolated Rabbit Heart

English Title

Voltage Sensitive Dye di-4-ANNEPS Prolongs Impulse Conduction Through Ventricles, but not Through AV Node in Isolated Rabbit Heart

Type

Paper in proceedings (conference paper)

Original Abstract

Voltage sensitive dyes are widely used for measurement of action potential in various cardiac studies. The elementary condition for its application is fact that measured electrophysiological parameters would not be affected by measuring procedure itself. The prolongation of RR interval in the presence of the most used voltage sensitive dye di-4-ANEPPS has been reported, however the exact mechanism is not known. In this study we examine impact of di-4-ANEPPS on impulse conduction through the atrioventricular (AV) node and cardiac ventricles in isolated rabbit heart. The impulse conduction through AV node in presence of di-4-ANEPPS was prolonged only in phase of dye loading at 14th, 16th and 17th minute. Only sporadic AV block was observed. In comparison, the prolongation of impulse conduction through cardiac ventricles was observed after 13th minute of dye loading and persisted to the end of this phase as well as over the whole wash out phase.

English abstract

Voltage sensitive dyes are widely used for measurement of action potential in various cardiac studies. The elementary condition for its application is fact that measured electrophysiological parameters would not be affected by measuring procedure itself. The prolongation of RR interval in the presence of the most used voltage sensitive dye di-4-ANEPPS has been reported, however the exact mechanism is not known. In this study we examine impact of di-4-ANEPPS on impulse conduction through the atrioventricular (AV) node and cardiac ventricles in isolated rabbit heart. The impulse conduction through AV node in presence of di-4-ANEPPS was prolonged only in phase of dye loading at 14th, 16th and 17th minute. Only sporadic AV block was observed. In comparison, the prolongation of impulse conduction through cardiac ventricles was observed after 13th minute of dye loading and persisted to the end of this phase as well as over the whole wash out phase.

Keywords

Voltage sensitive dye, di-4-ANEPPS, isolated heart, heart rate

Key words in English

Voltage sensitive dye, di-4-ANEPPS, isolated heart, heart rate

Authors

OLEJNÍČKOVÁ, V.; RONZHINA, M.; JANOUŠEK, O.; KOLÁŘOVÁ, J.; FIALOVÁ, K.; PROVAZNÍK, I.; NOVÁKOVÁ, M.

RIV year

2016

Released

01.11.2015

Location

Nice, Francie

ISBN

978-1-4799-4347-0

Book

Computing in Cardiology 2015

Pages from

1

Pages to

4

Pages count

4

Full text in the Digital Library

http://hdl.handle.net/

BibTex

@inproceedings{BUT118308,
  author="Veronika {Olejníčková} and Marina {Filipenská} and Oto {Janoušek} and Jana {Kolářová} and Kateřina {Fialová} and Valentýna {Provazník} and Marie {Nováková}",
  title="Voltage Sensitive Dye di-4-ANNEPS Prolongs Impulse Conduction Through Ventricles, but not Through AV Node in Isolated Rabbit Heart",
  booktitle="Computing in Cardiology 2015",
  year="2015",
  pages="1--4",
  address="Nice, Francie",
  isbn="978-1-4799-4347-0"
}