Publication result detail

Immunomagnetic capture and preconcentration of Lactobacillus cells

RITTICH, B.; TURKOVÁ, K.; TRACHTOVÁ, Š.; VAŇÁSEK, J.; HORÁK, D.; KOZÁKOVÁ H.; ŠPANOVÁ A.

Original Title

Immunomagnetic capture and preconcentration of Lactobacillus cells

English Title

Immunomagnetic capture and preconcentration of Lactobacillus cells

Type

Abstract

Original Abstract

The aim of this work was to prepare and evaluate imunomagnetic microspheres for the isolation of Lactobacillus cells from food matrices. Two types of magnetic particles coated by streptavidine were used: magnetic porous glass (MPG) (diameter 5 µm) (10 mg/mL, 4.6 x 107 particles/mL) (PureBiotech, USA) and 2.9 µm magnetic poly(glycidyl methacrylate) microspheres containing NH2 groups [P(GMA)-NH2]. They were functionalised with biotynilated anti-Lactobacillus antibody and used for separation of Lactobacillus cells. Preconcentration of cells in a milk product was also tested.

English abstract

The aim of this work was to prepare and evaluate imunomagnetic microspheres for the isolation of Lactobacillus cells from food matrices. Two types of magnetic particles coated by streptavidine were used: magnetic porous glass (MPG) (diameter 5 µm) (10 mg/mL, 4.6 x 107 particles/mL) (PureBiotech, USA) and 2.9 µm magnetic poly(glycidyl methacrylate) microspheres containing NH2 groups [P(GMA)-NH2]. They were functionalised with biotynilated anti-Lactobacillus antibody and used for separation of Lactobacillus cells. Preconcentration of cells in a milk product was also tested.

Keywords

Lactobacillus cells; immunomagnetic separation; food products

Key words in English

Lactobacillus cells; immunomagnetic separation; food products

Authors

RITTICH, B.; TURKOVÁ, K.; TRACHTOVÁ, Š.; VAŇÁSEK, J.; HORÁK, D.; KOZÁKOVÁ H.; ŠPANOVÁ A.

RIV year

2016

Released

24.05.2015

Publisher

Institute of Immunology and Experimental Therapy Polish Academy of Science

Location

Bielawa, Polsko

ISBN

978-83-928488-4-4

Book

2nd Polish-Czech Probiotics Conference: Microbiology, Immunology & Allergy,

Pages from

64

Pages to

64

Pages count

1

BibTex

@misc{BUT114813,
  author="RITTICH, B. and TURKOVÁ, K. and TRACHTOVÁ, Š. and VAŇÁSEK, J. and HORÁK, D. and KOZÁKOVÁ H. and ŠPANOVÁ A.",
  title="Immunomagnetic capture and preconcentration of Lactobacillus cells",
  booktitle="2nd Polish-Czech Probiotics Conference: Microbiology, Immunology & Allergy,",
  year="2015",
  edition="1",
  pages="64--64",
  publisher="Institute of Immunology and Experimental Therapy Polish Academy of Science",
  address="Bielawa, Polsko",
  isbn="978-83-928488-4-4",
  note="Abstract"
}