Publication result detail

The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses

CHUDOBOVÁ, D.; DOSTÁLOVÁ, S.; RUTTKAY-NEDECKÝ, B.; GURÁŇ, R.; MERLOS RODRIGO, M.; TMEJOVÁ, K.; KŘÍŽKOVÁ, S.; ZÍTKA, O.; ADAM, V.; KIZEK, R.

Original Title

The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses

English Title

The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses

Type

WoS Article

Original Abstract

tIn this study, we focused on the effect of heavy metal ions in resistant strains of gram-positive bacte-ria Staphylococcus aureus using biochemical methods and mass spectrometry. Five nitrate solutions ofheavy metals (Ag+, Cu2+, Cd2+, Zn2+ and Pb2+) were used to create S. aureus resistant strains. Biochem-ical changes of resistant strains in comparison with the non-resistant control strain of S. aureus wereobserved by microbiological (measuring - growth curves and inhibition zones) and spectrophotometricmethods (antioxidant activity and alaninaminotransferase, aspartateaminotransferase, alkaline phos-phatase, -glutamyltransferase activities). Mass spectrometry was employed for the qualitative analysisof the samples (changes in S. aureus protein composition) and for the identification of the strains databaseMALDI Biotyper was employed. Alterations, in terms of biochemical properties and protein composition,were observed in resistant strains compared to non-resistant control strain. Our results describe the pos-sible option for the analysis of S. aureus resistant strains and may thus serve as a support for monitoringof changes in genetic information caused by the forming of resistance to heavy metals.

English abstract

tIn this study, we focused on the effect of heavy metal ions in resistant strains of gram-positive bacte-ria Staphylococcus aureus using biochemical methods and mass spectrometry. Five nitrate solutions ofheavy metals (Ag+, Cu2+, Cd2+, Zn2+ and Pb2+) were used to create S. aureus resistant strains. Biochem-ical changes of resistant strains in comparison with the non-resistant control strain of S. aureus wereobserved by microbiological (measuring - growth curves and inhibition zones) and spectrophotometricmethods (antioxidant activity and alaninaminotransferase, aspartateaminotransferase, alkaline phos-phatase, -glutamyltransferase activities). Mass spectrometry was employed for the qualitative analysisof the samples (changes in S. aureus protein composition) and for the identification of the strains databaseMALDI Biotyper was employed. Alterations, in terms of biochemical properties and protein composition,were observed in resistant strains compared to non-resistant control strain. Our results describe the pos-sible option for the analysis of S. aureus resistant strains and may thus serve as a support for monitoringof changes in genetic information caused by the forming of resistance to heavy metals.

Keywords

S. aureus, Resistance, Heavy metals, Mass spectrometry

Key words in English

S. aureus, Resistance, Heavy metals, Mass spectrometry

Authors

CHUDOBOVÁ, D.; DOSTÁLOVÁ, S.; RUTTKAY-NEDECKÝ, B.; GURÁŇ, R.; MERLOS RODRIGO, M.; TMEJOVÁ, K.; KŘÍŽKOVÁ, S.; ZÍTKA, O.; ADAM, V.; KIZEK, R.

RIV year

2016

Released

01.01.2015

ISBN

0944-5013

Periodical

MICROBIOLOGICAL RESEARCH

Volume

170

Number

1

State

Federal Republic of Germany

Pages from

147

Pages to

156

Pages count

10

BibTex

@article{BUT113354,
  author="Dagmar {Hegerová} and Simona {Dostálová} and Branislav {Ruttkay-Nedecký} and Roman {Guráň} and Miguel Ángel {Merlos Rodrigo} and Kateřina {Tmejová} and Soňa {Křížková} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kizek}",
  title="The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses",
  journal="MICROBIOLOGICAL RESEARCH",
  year="2015",
  volume="170",
  number="1",
  pages="147--156",
  doi="10.1016/j.micres.2014.08.003",
  issn="0944-5013"
}