Publication result detail

TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA

Čuta R., Fričová M., Rittich B., Španová A.

Original Title

TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA

English Title

TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA

Type

Conference proceedings

Original Abstract

The aim of this work was the optimization of procedure the crude cell lysates preparation of Lactobacillus genus. In this experiment different lysis methods were tested: buffer B, 4% solution of washing powder Amway and using of immobilized lysozyme. At the same time a lysing reagent was tested. As a control sample was used a buffer A which contained no lysozyme. Crude lysates were used for a DNA isolation using magnetic microspheres. The DNA was examined by the PCR method.

English abstract

The aim of this work was the optimization of procedure the crude cell lysates preparation of Lactobacillus genus. In this experiment different lysis methods were tested: buffer B, 4% solution of washing powder Amway and using of immobilized lysozyme. At the same time a lysing reagent was tested. As a control sample was used a buffer A which contained no lysozyme. Crude lysates were used for a DNA isolation using magnetic microspheres. The DNA was examined by the PCR method.

Keywords

DNA, lysis, PCR

Key words in English

DNA, lysis, PCR

Authors

Čuta R., Fričová M., Rittich B., Španová A.

Released

01.10.2014

ISBN

978-80-904959-2-0

Pages from

170

Pages to

171

Pages count

2

BibTex

@proceedings{BUT111852,
  editor="Robert {Čuta} and Michaela {Čutová} and Bohuslav {Rittich} and Alena {Španová}",
  title="TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA",
  year="2014",
  pages="170--171",
  isbn="978-80-904959-2-0"
}