Publication result detail

Use of PCR-DGGE for control of bacteria in chesses and their pickles

ČAKAJDOVÁ, M.; TRACHTOVÁ, Š.; MOHELSKÝ, T.; NĚMEČKOVÁ, I.; ŠPANOVÁ, A.; RITTICH, B.

Original Title

Use of PCR-DGGE for control of bacteria in chesses and their pickles

English Title

Use of PCR-DGGE for control of bacteria in chesses and their pickles

Type

Conference proceedings

Original Abstract

Denaturation gradient gel electrophoresis (PCR-DGGE) was used for control of bacteria in brained cheeses and their pickles. Composition of polymerase chain reaction (PCR) mixture and DNA amplification conditions were optimized for PCR with CG clamp primers. Phenol extraction and magnetic microspheres were used for purification of amplicons. Different numbers and intensities of amplicons reflect different bacterial composition in tested cheeses and pickles.

English abstract

Denaturation gradient gel electrophoresis (PCR-DGGE) was used for control of bacteria in brained cheeses and their pickles. Composition of polymerase chain reaction (PCR) mixture and DNA amplification conditions were optimized for PCR with CG clamp primers. Phenol extraction and magnetic microspheres were used for purification of amplicons. Different numbers and intensities of amplicons reflect different bacterial composition in tested cheeses and pickles.

Keywords

DGGE, cheeses and their pickles

Key words in English

DGGE, cheeses and their pickles

Authors

ČAKAJDOVÁ, M.; TRACHTOVÁ, Š.; MOHELSKÝ, T.; NĚMEČKOVÁ, I.; ŠPANOVÁ, A.; RITTICH, B.

Released

20.10.2014

Publisher

Institute of Analytical Chemistry AS CR, v. v. i., Brno

Location

Brno, Czech Republic

ISBN

978-80-904959-2-0

Book

CECE 2014 11th International Interdisciplinary Meeting of Bioanalysis

Pages from

147

Pages to

150

Pages count

4

BibTex

@proceedings{BUT111768,
  editor="Martina {Čakajdová} and Štěpánka {Trachtová} and Tomáš {Mohelský} and Irena {Němečková} and Alena {Španová} and Bohuslav {Rittich}",
  title="Use of PCR-DGGE for control of bacteria in chesses and their pickles",
  year="2014",
  pages="147--150",
  publisher="Institute of Analytical Chemistry AS CR, v. v. i., Brno",
  address="Brno, Czech Republic",
  isbn="978-80-904959-2-0"
}