Publication result detail

Beads Based Electrochemical Assay for Detection of Hemagglutinin Labeled by Two Different Types of Cadmium Quantum Dots

KREJČOVÁ, L.; HYNEK, D.; GURÁŇ, R.; MICHÁLEK, P.; MOULICK, A.; KOPEL, P.; TMEJOVÁ, K.; NGUYEN, H.; ADAM, V.; HUBÁLEK, J.; KYNICKÝ, J.; KIZEK, R.

Original Title

Beads Based Electrochemical Assay for Detection of Hemagglutinin Labeled by Two Different Types of Cadmium Quantum Dots

English Title

Beads Based Electrochemical Assay for Detection of Hemagglutinin Labeled by Two Different Types of Cadmium Quantum Dots

Type

WoS Article

Original Abstract

In this study it is described beads based isolation assay terminated by electrochemical analysis of hemagglutinin (HA) using two different cadmium quantum dots (QDs) labelled as CdS and CdTe. The two steps assay was suggested as the first step was the automatic isolation of HA labelled by Cd QDs using glycan modified paramagnetic particles (MPs), and the second one was the electrochemical detection of HA-QDs complex by voltammetry, particularly, adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction was used for detection of HA and differential pulse anodic stripping voltammetry was used for detection of Cd. Both methods were optimized. Suggested assay can be used for the subtyping and quantification of HA.

English abstract

In this study it is described beads based isolation assay terminated by electrochemical analysis of hemagglutinin (HA) using two different cadmium quantum dots (QDs) labelled as CdS and CdTe. The two steps assay was suggested as the first step was the automatic isolation of HA labelled by Cd QDs using glycan modified paramagnetic particles (MPs), and the second one was the electrochemical detection of HA-QDs complex by voltammetry, particularly, adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction was used for detection of HA and differential pulse anodic stripping voltammetry was used for detection of Cd. Both methods were optimized. Suggested assay can be used for the subtyping and quantification of HA.

Keywords

Voltammetry; Paramagnetic Particles; Influenza Hemagglutinin; Influenza Vaccine; Quantum Dots;

Key words in English

Voltammetry; Paramagnetic Particles; Influenza Hemagglutinin; Influenza Vaccine; Quantum Dots;

Authors

KREJČOVÁ, L.; HYNEK, D.; GURÁŇ, R.; MICHÁLEK, P.; MOULICK, A.; KOPEL, P.; TMEJOVÁ, K.; NGUYEN, H.; ADAM, V.; HUBÁLEK, J.; KYNICKÝ, J.; KIZEK, R.

Released

01.07.2014

ISBN

1452-3981

Periodical

International Journal of Electrochemical Science

Volume

9

Number

7

State

Republic of Serbia

Pages from

3349

Pages to

3363

Pages count

15

BibTex

@article{BUT111181,
  author="Ludmila {Krejčová} and David {Hynek} and Roman {Guráň} and Petr {Michálek} and Amitava {Moulick} and Pavel {Kopel} and Kateřina {Tmejová} and Hoai Viet {Nguyen} and Vojtěch {Adam} and Jaromír {Hubálek} and Jindřich {Kynický} and René {Kizek}",
  title="Beads Based Electrochemical Assay for Detection of Hemagglutinin Labeled by Two Different Types of Cadmium Quantum Dots",
  journal="International Journal of Electrochemical Science",
  year="2014",
  volume="9",
  number="7",
  pages="3349--3363",
  issn="1452-3981"
}