Publication result detail

Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application

MANAKHOV, A.; SKLÁDAL, P.; NEČAS, D.; ČECHAL, J.; POLČÁK, J.; ELIÁŠ, M.; ZAJÍČKOVÁ, L.

Original Title

Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application

English Title

Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application

Type

WoS Article

Original Abstract

Biosensors have been extensively developed and applied for biomedical and environmental studies. Although there are many different types of biosensing techniques, all these methods require immobilization of biomolecules. Therefore, this is a critical issue in the development of any sort of biosensor affecting its operational properties. In this work, we address this critical issue by employing cyclopropylamine plasma polymerization to deposit stable amine thin films on the surface of quartz crystal microbalance (QCM) biosensors. The antibody specific to human serum albumin (anti-HSA) is attached to the QCM surface via cross-linkage obtained by intermediate reaction with glutaraldehyde. All steps of the bio-immobilization are controlled by XPS and FTIR to characterize surface and layer (bulk) chemistry. The results obtained with the antibody linked through the CPA plasma-polymerized layer are comparable with the results obtained previously when the same antibody AL-01 was linked to the surface using the self-assembled thiol monolayer based on cystamine.

English abstract

Biosensors have been extensively developed and applied for biomedical and environmental studies. Although there are many different types of biosensing techniques, all these methods require immobilization of biomolecules. Therefore, this is a critical issue in the development of any sort of biosensor affecting its operational properties. In this work, we address this critical issue by employing cyclopropylamine plasma polymerization to deposit stable amine thin films on the surface of quartz crystal microbalance (QCM) biosensors. The antibody specific to human serum albumin (anti-HSA) is attached to the QCM surface via cross-linkage obtained by intermediate reaction with glutaraldehyde. All steps of the bio-immobilization are controlled by XPS and FTIR to characterize surface and layer (bulk) chemistry. The results obtained with the antibody linked through the CPA plasma-polymerized layer are comparable with the results obtained previously when the same antibody AL-01 was linked to the surface using the self-assembled thiol monolayer based on cystamine.

Keywords

amine plasma; biosensors; polymers; quartz crystal microbalance; X-ray photoelectron spectroscopy

Key words in English

amine plasma; biosensors; polymers; quartz crystal microbalance; X-ray photoelectron spectroscopy

Authors

MANAKHOV, A.; SKLÁDAL, P.; NEČAS, D.; ČECHAL, J.; POLČÁK, J.; ELIÁŠ, M.; ZAJÍČKOVÁ, L.

Released

12.12.2014

ISBN

1862-6300

Periodical

PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE

Volume

211

Number

12

State

Federal Republic of Germany

Pages from

2801

Pages to

2808

Pages count

8

BibTex

@article{BUT111156,
  author="Anton {Manakhov} and Petr {Skládal} and David {Nečas} and Jan {Čechal} and Josef {Polčák} and Marek {Eliáš} and Lenka {Zajíčková}",
  title="Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application",
  journal="PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE",
  year="2014",
  volume="211",
  number="12",
  pages="2801--2808",
  doi="10.1002/pssa.201431399",
  issn="1862-6300"
}