Publication result detail

Succession and Replacement of Bacterial Populations in the Caecum of Egg Laying Hens over Their Whole Life

VÍDEŇSKÁ, P.; SEDLÁŘ, K.; LUKAC, M.; FALDYNOVÁ, M.; GERŽOVÁ, L.; ČEJKOVÁ, D.; ŠIŠÁK, F.; RYCHLÍK, I.

Original Title

Succession and Replacement of Bacterial Populations in the Caecum of Egg Laying Hens over Their Whole Life

English Title

Succession and Replacement of Bacterial Populations in the Caecum of Egg Laying Hens over Their Whole Life

Type

WoS Article

Original Abstract

In this study we characterised the development of caecal microbiota in egg laying hens over their commercial production lifespan, from the day of hatching until 60 weeks of age. Using pyrosequencing of V3/V4 variable regions of 16S rRNA genes for microbiota characterisation, we were able to define 4 different stages of caecal microbiota development. The first stage lasted for the first week of life and was characterised by a high prevalence of Enterobacteriaceae (phylum Proteobacteria). The second stage lasted from week 2 to week 4 and was characterised by nearly an absolute dominance of Lachnospiraceae and Ruminococcaceae (both phylum Firmicutes). The third stage lasted from month 2 to month 6 and was characterised by the succession of Firmicutes at the expense of Bacteroidetes. The fourth stage was typical for adult hens in full egg production aged 7 months or more and was characterised by a constant ratio of Bacteroidetes and Firmicutes formed by equal numbers of the representatives of both phyla.

English abstract

In this study we characterised the development of caecal microbiota in egg laying hens over their commercial production lifespan, from the day of hatching until 60 weeks of age. Using pyrosequencing of V3/V4 variable regions of 16S rRNA genes for microbiota characterisation, we were able to define 4 different stages of caecal microbiota development. The first stage lasted for the first week of life and was characterised by a high prevalence of Enterobacteriaceae (phylum Proteobacteria). The second stage lasted from week 2 to week 4 and was characterised by nearly an absolute dominance of Lachnospiraceae and Ruminococcaceae (both phylum Firmicutes). The third stage lasted from month 2 to month 6 and was characterised by the succession of Firmicutes at the expense of Bacteroidetes. The fourth stage was typical for adult hens in full egg production aged 7 months or more and was characterised by a constant ratio of Bacteroidetes and Firmicutes formed by equal numbers of the representatives of both phyla.

Keywords

metagenomics, chicken microbiota, 16S rRNA

Key words in English

metagenomics, chicken microbiota, 16S rRNA

Authors

VÍDEŇSKÁ, P.; SEDLÁŘ, K.; LUKAC, M.; FALDYNOVÁ, M.; GERŽOVÁ, L.; ČEJKOVÁ, D.; ŠIŠÁK, F.; RYCHLÍK, I.

RIV year

2015

Released

12.12.2014

Publisher

Public Library of Science

Location

California (US)

ISBN

1932-6203

Periodical

PLoS One

Volume

9

Number

12

State

United States of America

Pages from

1

Pages to

14

Pages count

14

URL

http://europepmc.org/articles/PMC4264878?pdf=render

Full text in the Digital Library

http://hdl.handle.net/11012/69206

BibTex

@article{BUT111135,
  author="Petra {Vídeňská} and Karel {Sedlář} and Maja {Lukac} and Marcela {Faldynová} and Lenka {Geržová} and Darina {Čejková} and František {Šišák} and Ivan {Rychlík}",
  title="Succession and Replacement of Bacterial Populations in the Caecum of Egg Laying Hens over Their Whole Life",
  journal="PLoS One",
  year="2014",
  volume="9",
  number="12",
  pages="1--14",
  doi="10.1371/journal.pone.0115142",
  issn="1932-6203",
  url="http://europepmc.org/articles/PMC4264878?pdf=render"
}

Documents

journal.pone.0115142