Detail publikačního výsledku

Precise Localization and Quantification of Magnetic/Fluorescent Nanoparticles Inside Mesenchymal Stromal Cells by Confocal Microscopy

POLÁKOVÁ, K.; ČMIEL, V.; SKOPALÍK, J.; HAVRDOVÁ, M.; MAGRO, M.; SOLAŘ, J.; ZBOŘIL, R.

Original Title

Precise Localization and Quantification of Magnetic/Fluorescent Nanoparticles Inside Mesenchymal Stromal Cells by Confocal Microscopy

English Title

Precise Localization and Quantification of Magnetic/Fluorescent Nanoparticles Inside Mesenchymal Stromal Cells by Confocal Microscopy

Type

Abstract

Original Abstract

In vivo tracking of magnetically labeled stem cells by non-invasive Magnetic Resonance Imaging (MRI) is used as an up to date method of diagnosis after transplantation. The process in vitro is tested on the cell level using confocal microscope after addition of SPIO (Superparamagnetic Iron Oxide) nanoparticles into the cultivation medium that pass through the cell membrane. Nanoparticles with organic dyes enable effective tracking in organells inside MCS. The poster describes the nanoparticles localization using Matlab.

English abstract

In vivo tracking of magnetically labeled stem cells by non-invasive Magnetic Resonance Imaging (MRI) is used as an up to date method of diagnosis after transplantation. The process in vitro is tested on the cell level using confocal microscope after addition of SPIO (Superparamagnetic Iron Oxide) nanoparticles into the cultivation medium that pass through the cell membrane. Nanoparticles with organic dyes enable effective tracking in organells inside MCS. The poster describes the nanoparticles localization using Matlab.

Keywords

MCS, mezenchymal cells, confocal microscopy, SPIO

Key words in English

MCS, mezenchymal cells, confocal microscopy, SPIO

Authors

POLÁKOVÁ, K.; ČMIEL, V.; SKOPALÍK, J.; HAVRDOVÁ, M.; MAGRO, M.; SOLAŘ, J.; ZBOŘIL, R.

Released

05.11.2014

Full text in the Digital Library

http://hdl.handle.net/

BibTex

@misc{BUT110529,
  author="Kateřina {Poláková} and Vratislav {Čmiel} and Josef {Skopalík} and Markéta {Havrdová} and Maxmiliano {Magro} and Jan {Solař} and Radek {Zbořil}",
  title="Precise Localization and Quantification of Magnetic/Fluorescent Nanoparticles Inside Mesenchymal Stromal Cells by Confocal Microscopy",
  year="2014",
  note="Abstract"
}