Publication result detail

Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns

ŠESTÁK, J.; KAHLE, V.

Original Title

Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns

English Title

Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns

Type

WoS Article

Original Abstract

In this short communication, a low cost and easily feasible pressure-controlled extension of the previously described simple gradient liquid chromatography platform is proposed. A test mixture of four nitro esters was separated by 10-60%v/v acetone/water gradient and a high repeatability of retention volumes at 20 MPa (RSD less than 0.45%) was realized. Separations were also performed at different values of pressure (20, 25, and 31 MPa), and only small variations of the retention volumes (up to 0.8%) were observed. In this particular case, the gain in the analysis speed of 7% compared to the constant flow mode was realized at a constant pressure.

English abstract

In this short communication, a low cost and easily feasible pressure-controlled extension of the previously described simple gradient liquid chromatography platform is proposed. A test mixture of four nitro esters was separated by 10-60%v/v acetone/water gradient and a high repeatability of retention volumes at 20 MPa (RSD less than 0.45%) was realized. Separations were also performed at different values of pressure (20, 25, and 31 MPa), and only small variations of the retention volumes (up to 0.8%) were observed. In this particular case, the gain in the analysis speed of 7% compared to the constant flow mode was realized at a constant pressure.

Keywords

Constant pressure HPLC; Gradient elution; Simple liquid chromatograph; Nano columns; Nitro esters

Key words in English

Constant pressure HPLC; Gradient elution; Simple liquid chromatograph; Nano columns; Nitro esters

Authors

ŠESTÁK, J.; KAHLE, V.

RIV year

2015

Released

11.06.2014

ISBN

0021-9673

Periodical

JOURNAL OF CHROMATOGRAPHY A

Volume

1350

Number

6

State

Kingdom of the Netherlands

Pages from

68

Pages to

71

Pages count

4

BibTex

@article{BUT107541,
  author="Jozef {Šesták} and Vladislav {Kahle}",
  title="Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns",
  journal="JOURNAL OF CHROMATOGRAPHY A",
  year="2014",
  volume="1350",
  number="6",
  pages="68--71",
  issn="0021-9673"
}