Publication result detail

Influence of water environment on biological specimens imaging in ESEM

AUTRATA, R.; NEDĚLA, V.; HORKÝ, D.

Original Title

Influence of water environment on biological specimens imaging in ESEM

English Title

Influence of water environment on biological specimens imaging in ESEM

Type

Paper in proceedings (conference paper)

Original Abstract

Environmental scanning electron microscope (ESEM) allows the visualisation of specimens that are difficult or impossible to image in a conventional high vacuum SEM. Pressure in the specimen chamber ranges from 1 Pa over 2000 Pa, which permits to observe specimens of beeing free from charging artefacts and containing different volume of water. But there are some difficulties with the observation of highly wet biological specimens in their natural state because during a pre-pumping process of the specimen chamber an escape of water from the specimen occurs and thus its surface is damaged. The aim of this contribution is to find a method making possible to decrease water escape from the specimen during the initiation pumping process of the ESEM, immediately after the inserting of the specimen into the specimen chamber and during an image recording.

English abstract

Environmental scanning electron microscope (ESEM) allows the visualisation of specimens that are difficult or impossible to image in a conventional high vacuum SEM. Pressure in the specimen chamber ranges from 1 Pa over 2000 Pa, which permits to observe specimens of beeing free from charging artefacts and containing different volume of water. But there are some difficulties with the observation of highly wet biological specimens in their natural state because during a pre-pumping process of the specimen chamber an escape of water from the specimen occurs and thus its surface is damaged. The aim of this contribution is to find a method making possible to decrease water escape from the specimen during the initiation pumping process of the ESEM, immediately after the inserting of the specimen into the specimen chamber and during an image recording.

Keywords

biological specimens, imaging, EREM

Key words in English

biological specimens, imaging, EREM

Authors

AUTRATA, R.; NEDĚLA, V.; HORKÝ, D.

Released

01.01.2003

Location

Pula

Book

Proceedings 6th Multinational Congress on Microscopy

Pages from

497

Pages count

2

URL

ÚPT AV ČR Brno

BibTex

@inproceedings{BUT10735,
  author="Rudolf {Autrata} and Vilém {Neděla} and Drahomír {Horký}",
  title="Influence of water environment on biological specimens imaging in ESEM",
  booktitle="Proceedings 6th Multinational Congress on Microscopy",
  year="2003",
  number="1",
  pages="2",
  address="Pula",
  url="ÚPT AV ČR Brno"
}