Publication result detail

DNA interaction with zinc(II) ions

NEJDL, L.; RUTTKAY-NEDECKÝ, B.; KUDR, J.; KŘÍŽKOVÁ, S.; ŠMERKOVÁ, K.; DOSTÁLOVÁ, S.; VACULOVIČOVÁ, M.; KOPEL, P.; ZEHNÁLEK, J.; TRNKOVÁ, L.; BABULA, P.; ADAM, V.; KIZEK, R.

Original Title

DNA interaction with zinc(II) ions

English Title

DNA interaction with zinc(II) ions

Type

WoS Article

Original Abstract

We focused on interactions of Zn(II) with DNA in this study. These interactions were monitored usingUV/vis spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified 498 bp fragmentof DNA. Samples were obtained by incubation of DNA fragment with Zn(II) for 60 min at 25 C. After incubation, the samples were dialyzed and analyzed immediately. In this way, DNA was converted intoa metal bound DNA (Zn–DNA). Interaction of Zn(II) with DNA caused change in the absorption spectrum(190–350 nm) and decrease in the melting temperature (Tm) of Zn–DNA. Spectrophotometric (UV/vis)analysis showed that increasing concentrations of zinc(II) ions led to the increase in the absorbance at200 nm and decrease in absorbance at 251 nm.

English abstract

We focused on interactions of Zn(II) with DNA in this study. These interactions were monitored usingUV/vis spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified 498 bp fragmentof DNA. Samples were obtained by incubation of DNA fragment with Zn(II) for 60 min at 25 C. After incubation, the samples were dialyzed and analyzed immediately. In this way, DNA was converted intoa metal bound DNA (Zn–DNA). Interaction of Zn(II) with DNA caused change in the absorption spectrum(190–350 nm) and decrease in the melting temperature (Tm) of Zn–DNA. Spectrophotometric (UV/vis)analysis showed that increasing concentrations of zinc(II) ions led to the increase in the absorbance at200 nm and decrease in absorbance at 251 nm.

Keywords

UV/vis spectrophotometry, Gel electrophoresis, Melting temperature

Key words in English

UV/vis spectrophotometry, Gel electrophoresis, Melting temperature

Authors

NEJDL, L.; RUTTKAY-NEDECKÝ, B.; KUDR, J.; KŘÍŽKOVÁ, S.; ŠMERKOVÁ, K.; DOSTÁLOVÁ, S.; VACULOVIČOVÁ, M.; KOPEL, P.; ZEHNÁLEK, J.; TRNKOVÁ, L.; BABULA, P.; ADAM, V.; KIZEK, R.

Released

01.03.2014

ISBN

0141-8130

Periodical

International Journal of Biological Macromolecules

Volume

64

Number

3

State

Kingdom of the Netherlands

Pages from

281

Pages to

287

Pages count

7

BibTex

@article{BUT106908,
  author="Lukáš {Nejdl} and Branislav {Ruttkay-Nedecký} and Jiří {Kudr} and Soňa {Křížková} and Kristýna {Šmerková} and Simona {Dostálová} and Markéta {Vaculovičová} and Pavel {Kopel} and Josef {Zehnálek} and Libuše {Trnková} and Petr {Babula} and Vojtěch {Adam} and René {Kizek}",
  title="DNA interaction with zinc(II) ions",
  journal="International Journal of Biological Macromolecules",
  year="2014",
  volume="64",
  number="3",
  pages="281--287",
  doi="10.1016/j.ijbiomac.2013.12.013",
  issn="0141-8130"
}