Publication result detail

Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis

KUKAČKA, J.; HÚSKA, D.; POUCH, M.; KŘÍŽKOVÁ, S.; DRBOHLAVOVÁ, J.; CHOMOUCKÁ, J.; ADAM, V.; HUBÁLEK, J.; KLAPKOVÁ, E.; PRŮŠA, R.; KIZEK, R.

Original Title

Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis

English Title

Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis

Type

Abstract

Original Abstract

In this study, magnetic nanobeads coated with streptavidine were prepared to isolate ssDNA specific to Hepatitis B virus (HBV). DNA probes labeled by biotine molecules were designed to be complementary to specific HBV genes. Artificial synthetic oligonucleotides (25–35 nt), identical to HBV gene sequences, were used to mimic a real sample of human blood serum. The amount of nanoparticles utilized for isolation target molecules was within the range from 1 to 20 uL. It was found that already 1 uL of MNPs was sufficient for effective isolation of the molecule of the interest.

English abstract

In this study, magnetic nanobeads coated with streptavidine were prepared to isolate ssDNA specific to Hepatitis B virus (HBV). DNA probes labeled by biotine molecules were designed to be complementary to specific HBV genes. Artificial synthetic oligonucleotides (25–35 nt), identical to HBV gene sequences, were used to mimic a real sample of human blood serum. The amount of nanoparticles utilized for isolation target molecules was within the range from 1 to 20 uL. It was found that already 1 uL of MNPs was sufficient for effective isolation of the molecule of the interest.

Keywords

magnetic nanobeads, chip electrophoresis

Key words in English

magnetic nanobeads, chip electrophoresis

Authors

KUKAČKA, J.; HÚSKA, D.; POUCH, M.; KŘÍŽKOVÁ, S.; DRBOHLAVOVÁ, J.; CHOMOUCKÁ, J.; ADAM, V.; HUBÁLEK, J.; KLAPKOVÁ, E.; PRŮŠA, R.; KIZEK, R.

RIV year

2014

Released

01.04.2011

Publisher

FEDERATION AMER SOC EXP BIOL

Location

BETHESDA, MD 20814-3998 USA

ISBN

1530-6860

Periodical

FASEB JOURNAL

Volume

25

State

United States of America

BibTex

@misc{BUT105322,
  author="Jiří {Kukačka} and Dalibor {Húska} and Milan {Pouch} and Soňa {Křížková} and Jana {Drbohlavová} and Jana {Pekárková} and Vojtěch {Adam} and Jaromír {Hubálek} and Eva {Klapková} and Richard {Průša} and René {Kizek}",
  title="Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis",
  year="2011",
  journal="FASEB JOURNAL",
  volume="25",
  publisher="FEDERATION AMER SOC EXP BIOL",
  address="BETHESDA, MD 20814-3998 USA",
  issn="0892-6638",
  note="Abstract"
}