Detail publikačního výsledku

Electrophoretic study of peptide-mediated quantum dot-human immunoglobulin bioconjugation

JANŮ, L.; STANISAVLJEVIC, M.; KŘÍŽKOVÁ, S.; ŠOBROVÁ, P.; VACULOVIČOVÁ, M.; KIZEK, R.; ADAM, V.

Original Title

Electrophoretic study of peptide-mediated quantum dot-human immunoglobulin bioconjugation

English Title

Electrophoretic study of peptide-mediated quantum dot-human immunoglobulin bioconjugation

Type

WoS Article

Original Abstract

The bioconjugation of quantum dots (QDs) is a key process in their application for bioanalysis as well as imaging. The coupling of QDs with biologically active molecules such as peptides, nucleic acids, and/or antibodies enables their fluorescent labeling, and therefore, selective and sensitive tracking during the bioanalytical process, however, the efficiency of the labeling and preservation of the biological activity of the bioconjugate have to be considered. In this study, a new approach of the bioconjugation of CdTe-QDs and human immunoglobulin employing a small peptide is described

English abstract

The bioconjugation of quantum dots (QDs) is a key process in their application for bioanalysis as well as imaging. The coupling of QDs with biologically active molecules such as peptides, nucleic acids, and/or antibodies enables their fluorescent labeling, and therefore, selective and sensitive tracking during the bioanalytical process, however, the efficiency of the labeling and preservation of the biological activity of the bioconjugate have to be considered. In this study, a new approach of the bioconjugation of CdTe-QDs and human immunoglobulin employing a small peptide is described

Keywords

Antibody, Bioconjugation, Gel electrophoresis, Immunoglobulin, Quantum dot

Key words in English

Antibody, Bioconjugation, Gel electrophoresis, Immunoglobulin, Quantum dot

Authors

JANŮ, L.; STANISAVLJEVIC, M.; KŘÍŽKOVÁ, S.; ŠOBROVÁ, P.; VACULOVIČOVÁ, M.; KIZEK, R.; ADAM, V.

RIV year

2014

Released

01.09.2013

ISBN

0173-0835

Periodical

ELECTROPHORESIS

Volume

34

Number

18

State

Federal Republic of Germany

Pages from

2725

Pages to

2732

Pages count

8

Full text in the Digital Library

http://hdl.handle.net/

BibTex

@article{BUT103146,
  author="Libor {Janů} and Maja {Stanisavljevic} and Soňa {Křížková} and Pavlína {Adam} and Markéta {Vaculovičová} and René {Kizek} and Vojtěch {Adam}",
  title="Electrophoretic study of peptide-mediated quantum dot-human immunoglobulin bioconjugation",
  journal="ELECTROPHORESIS",
  year="2013",
  volume="34",
  number="18",
  pages="2725--2732",
  doi="10.1002/elps.201300088",
  issn="0173-0835"
}