Publication result detail

Effect of sarcosine on antioxidant parameters and metallothionein content in the PC-3 prostate cancer cell line

CERNEI, N.; ZÍTKA, O.; SKALIČKOVÁ, S.; GUMULEC, J.; SZTALMACHOVÁ, M.; MERLOS RODRIGO, M.; SOCHOR, J.; MASAŘÍK, M.; ADAM, V.; HUBÁLEK, J.; TRNKOVÁ, L.; KRUSEOVÁ, J.; ECKSCHLAGER, T.; KIZEK, R.

Original Title

Effect of sarcosine on antioxidant parameters and metallothionein content in the PC-3 prostate cancer cell line

English Title

Effect of sarcosine on antioxidant parameters and metallothionein content in the PC-3 prostate cancer cell line

Type

WoS Article

Original Abstract

Sarcosine is currently one of the most discussed markers of prostate cancer. It is involved in amino acid metabolism and methylation processes that occur during the progression of prostate cancer. In this study, we monitored the effect of the addition of sarcosine (0; 10; 250; 500; 1,000 and 1,500 uM) in a time-dependent manner (0-72 h) on the PC-3 prostate cancer cell line. For the assessment of cell viability, the commonly used MTT test was employed. Furthermore, ion-exchange liquid chromatography was used for the determination of sarcosine content in the PC-3 cells

English abstract

Sarcosine is currently one of the most discussed markers of prostate cancer. It is involved in amino acid metabolism and methylation processes that occur during the progression of prostate cancer. In this study, we monitored the effect of the addition of sarcosine (0; 10; 250; 500; 1,000 and 1,500 uM) in a time-dependent manner (0-72 h) on the PC-3 prostate cancer cell line. For the assessment of cell viability, the commonly used MTT test was employed. Furthermore, ion-exchange liquid chromatography was used for the determination of sarcosine content in the PC-3 cells

Keywords

sarcosine, prostate cancer, metallothionein, antioxidant activity, chip electrophoresis, ion-exchange chromatography, Brdicka reaction

Key words in English

sarcosine, prostate cancer, metallothionein, antioxidant activity, chip electrophoresis, ion-exchange chromatography, Brdicka reaction

Authors

CERNEI, N.; ZÍTKA, O.; SKALIČKOVÁ, S.; GUMULEC, J.; SZTALMACHOVÁ, M.; MERLOS RODRIGO, M.; SOCHOR, J.; MASAŘÍK, M.; ADAM, V.; HUBÁLEK, J.; TRNKOVÁ, L.; KRUSEOVÁ, J.; ECKSCHLAGER, T.; KIZEK, R.

RIV year

2014

Released

01.06.2013

ISBN

1021-335X

Periodical

ONCOLOGY REPORTS

Volume

29

Number

6

State

Hellenic Republic

Pages from

2459

Pages to

2466

Pages count

8

BibTex

@article{BUT100725,
  author="Natalia Vladimirovna {Cernei} and Ondřej {Zítka} and Sylvie {Skaličková} and Jaromír {Gumulec} and Markéta {Sztalmachová} and Miguel Ángel {Merlos Rodrigo} and Jiří {Sochor} and Michal {Masařík} and Vojtěch {Adam} and Jaromír {Hubálek} and Libuše {Trnková} and Jarmila {Kruseová} and Tomáš {Eckschlager} and René {Kizek}",
  title="Effect of sarcosine on antioxidant parameters and metallothionein content in the PC-3 prostate cancer cell line",
  journal="ONCOLOGY REPORTS",
  year="2013",
  volume="29",
  number="6",
  pages="2459--2466",
  doi="10.3892/or.2013.2389",
  issn="1021-335X"
}