Sepsis remains the leading cause of mortality in the intensive care units. Immune system plays key role in sepsis development and control. Current knowledge of immune response cannot fully explain the mechanisms of cellular dysfunction and organ failure in sepsis and did not provide sufficient therapy options to slow down the sepsis progress. Peripheral blood monocytes have central role in sepsis and are source of numerous effector moleculs as well as diagnostic or prognostic markers. The main aim of the project is to dissect unresolved issues of monocytes signaling during sepsis including the crosstalk of major transcription factors (NFkB, HIF-1a and NFAT) and understands their role in expression of sepsis markers. We plan to analyse patient isolated monocytes as well as newly developed 3D tissue culture models allowing to culture monocytes in presence of lung tissue. Furthermore volatile substances from serum samples will be analysed to detect important metabolism changes related to innate cells exuberant activation. Taken together the project will provide better understanding of major processes controlling monocyte fate in sepsis as well as propose timely validation of sepsis markers. Note: In case, that the project will be funded, the main investigator for CEITEC VUT will be Ing. Lucie Pompeiano Vaníčková, Ph.D.

Keywords
spesis;monocytes;markers

NV18-06-00529

English

Pompeiano Vaníčková Lucie, Ing., Ph.D. - principal person responsible

Smart Nanodevices
- responsible department (25.6.2017 - not assigned)
Smart Nanodevices
- co-beneficiary (25.6.2017 - not assigned)

ZEMÁNKOVÁ, K.; PAVELICOVÁ, K.; POMPEIANO, A.; MRAVCOVÁ, L.; VACULOVIČOVÁ, M.; POMPEIANO VANÍČKOVÁ, L. Targeted volatolomics of human monocytes: Comparison of 2D-GC/TOF-MS and 1D-GC/Orbitrap-MS methods. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2021, vol. 1184, no. 122975, p. 1-10. ISSN: 1570-0232.
Detail

Responsibility: Pompeiano Vaníčková Lucie, Ing., Ph.D.