Course detail

Laboratory Classes in Instrumental Analysis - S2

FCH-MC_IA2_PAcad. year: 2023/2024

The subject aims at practical demostration of analytical techniques which are used in the analysis of materials, biological systems, transport systems for biologically active substances and in technical practice.

Language of instruction


Number of ECTS credits


Mode of study

Not applicable.

Entry knowledge

Knowledge of general, inorganic, organic, physical, analytical chemistry and biochemistry.

Rules for evaluation and completion of the course

The resulting evaluation is the average of the evaluation of individual protocols.
Mandatory participation in all blaboratory classes.


The aim of the subject is Improvement of knowledge gained during lectures and familiarize students with methods of instrumental and structural analysis used in the field of chemistry of biologically active substances.
Gaining practical knowledge in the field of analysis of biological samples, selection of the apropriate method, evaluation and interpretation of the results.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Klouda P.: Moderní analytické metody. Nakladatelství Pavel Klouda, Ostrava 2003. (CS)
Študlík a kolektiv: Analytické separační metody. Nakladatelství Karolinum, Praha 2004 (CS) (CS)
Králová a kolektiv: Bioanalytické metody. Vysoká škola chemicko-technologická v Praze (CS) (CS)

Recommended reading

Not applicable.


Classification of course in study plans

  • Programme NPCP_CHMA Master's

    specialization CHBL , 1. year of study, winter semester, compulsory

Type of course unit


Guided consultation in combined form of studies

26 hours, obligation not entered

Teacher / Lecturer

Laboratory exercise

39 hours, compulsory

Teacher / Lecturer


1. Introduction
2. Liquid chromatography for analysis of medical drugs
3. LC-MS analysis of biological samples
4. Analysis of volatile compounds by gas chromatography
5. UV-VIS spectroscopy as a tool for analysis of enzymes
6. FTIR spectroscopy for analysis of biopolymers
7. Fluorescence spectroscopy as method for analysis of transport of biologically active samples
8. Fluoresecence microscopy analysis at single cell level
9. Gel electrophoresis
10. Determination of fermentation products by CZE
11. Analysis of heavy meatl contamination by ICP-MS
12. Flow cytometry to analysis of cell populations
13. ELISA instrumentace and assays