Microscopic Imaging Technology
FEKT-MPC-MZTAcad. year: 2022/2023
The course will be a detailed overview of the principle and practice of light microscopy. The emphasis of the course will be on the correct and appropriate use of the light microscope. Course covers optical microscope theory and also advanced optical and imaging techniques.
Learning outcomes of the course unit
- describe spatial transfer of the electromagnetic wave,
- list and explain meaning of its parameters,
- define the main optical laws,
- apply Fresnel coefficients for specific case,
- describe simple optical system by matrix notation,
- describe the principle of light microscope,
- discuss the function of specific microscopy components,
- revise basic optical abberations and their influence on image quality,
- compare the properties of polarization, dark-field, phase and Nomarsky contrast microscopy techniques,
- describe the fluorescence microscope and its application,
- describe the confocal microscope and its application,
- explain physical principle of two-photon microscopy,
- explain TIRF microscopy,
- discuss, compare and select the optimal light detecion method,
- choose appropriate microscopy technique for specific application.
Recommended optional programme components
P. Mouroulis Visual Instrumentation, McGraw-Hill, 1999
J. Kuběna, Úvod do optiky, MU Brno 1994, skriptum
Murphy,D.B.: Fundamentals of light microscopy and electronic imaging, Wiley, 2011 (CS)
Sharma, K.K.: Optics: Principles and Applications, Academic Press, 2006 (CS)
Planned learning activities and teaching methods
Assesment methods and criteria linked to learning outcomes
Final exam: 0 - 60 points
Final exam is focused on testing the knowledge from the light microscopy imaging.
Language of instruction
2. Fresnel coefficients.
3. Matrix description of the optic systems.
4. Eye as an optical system.
5. Basic microscopy design concept.
6. CCD, CMOS sensors, photomultiplier, Avalanche diode, hybrid detectors its principle and parameters.
7. Dark field microscopy.
8. Phase contrast microscopy.
10. Nomarsky differential interference contrast microscopy.
11. Hoffman modulation contrast microscopy.
12. Fluorescence microscopy.
13. Laser scanning (confocal) microscopy.
14. 2-photon and multi-photon microscopy. TIRF microscopy.
15. Optical coherent microscopy.
16. Selected fluorescence microscopic techniques.
17. Advanced and superresolution techniques - STED, STORM/PALM, SIM.
18. Basic of electron microscopy.
Specification of controlled education, way of implementation and compensation for absences
Classification of course in study plans
- Programme MPC-BTB Master's, 2. year of study, winter semester, 5 credits, compulsory