Course detail

Laboratory Classes in Instrumental and Structure Analysis - FSBT

FCH-MCA_ISA_PAcad. year: 2022/2023

Practical class to the lecture Instrumental and Structure Analysis.
Students are familiarized with instrumental analytical techniques used by control and analysis of natural substances during this class. The course practically develops knowledge from the subjects Analytical Chemistry II and Instrumental and Structural Analysis. Individual tasks and laboratory equipment were chosen so that students come into direct contact with modern infrastructure in solving specific problems of analysis of samples of natural substances. Practical exercises are focused on separation techniques (chromatography and electrophoresis), elemental analysis, spectral and structural analysis.

Language of instruction


Number of ECTS credits


Mode of study

Not applicable.

Learning outcomes of the course unit

Graduates of this practical subject will acquire basic knowledge about selected instrumental analytical methods which they can may encounter in food chemistry practice. Student will learn basics of operation, maintanance and fuction principles of specific instruments. Furthermore acquire the skills of data evalutaion.


Successful completion of the practical class is conditional on the basic knowledge of the lecture Instrumental and Structure Analysis.


Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Laboratory exercise - 3 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.
Teaching in the distance form is performed in the block form on the basis of individual agreement between teachers and students.

Assesment methods and criteria linked to learning outcomes

Practical exercises are completed by classified credit. 100% present participation and submited laboratory diaries with protocols is neccesery for the succesful completion of the exercises. Absence can be excused only on the basis of a medical certificate. Final score is based on the average of grades of individual laboratory protocols.

Course curriculum

During the laboratory classes, these tasks are taught:

1. Optical emmision spectrometry with inductively coupled plasma - ICP-OES
2. Mass spectrometry with inductively coupled plasma - ICP-MS
3. Electromigration methods - CZE
4. Electromigration methods - chip gel electrophoresis
5. Liquid chromatography - HPLC
6. Liquid chromatography with mass detection - LC-MS
7. Gas chromatography - GC
8. Gas chromatography with mass detection - GC-MS
9. UV-VIS Spectrophotometry - UV-VIS
10. Infrared spectroscopy – IR

Work placements

Not applicable.


The goal of the laboratory classes is to improve theoretical and practical knowledge about instrumental analytical methods, focused on analysis of natural substances.

Specification of controlled education, way of implementation and compensation for absences

The full attendance is required. In the case of absence, the particular seminar can be compensated by attending with another group or in the last week of semester. An unexcused absence is a reason for the loss of the course-unit credit.

The examination of basic knowledge relating to the particular project is done at the beginning of each exercise. Each student will write out laboratory diary. Results from the practise will be summarized as hand-writen laboratory protocol, which will be part of laboratory diary.
Laboratory reports are required till 7 days after the last exercise. Classification of the student will be based on evaluation of laboratory diary, protocols and activity in laboratory.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

E-learningový kurz se souborem návodů a interaktivních názorných podpor. (CS)
Skoog D., West D.M., Holler F.J., Crouch S.R.: Analytická chemie, ČVUT, Praha, Česká republika, 2019. (CS)
Nováková L., Douša M. a kol.: Moderní HPLC separace v teorii a praxi I, Europrint, Praha, Česká republika, 2013. (CS)
Nováková L., Douša M. a kol.: Moderní HPLC separace v teorii a praxi II, Europrint, Praha, Česká republika, 2013. (CS)
Mikuš P., Piešťanský J., Dokupilová S.: Kvapalinová chromatografia, hmotnosná spektrometria, Veda, Bratislava, Slovensko, 2018. (SK)
Milata V.: Vybrané metódy molekulovej spektroskopie, STU, Bratislava, Slovensko, 2007. (SK)

Recommended reading

Robinson J.W. a kol.:Instrumental Analytical Chemistry: An Introduction, Taylor & Francis Ltd, London, UK, 2017. (EN)

Classification of course in study plans

  • Programme NPCP_CHTP Master's

    branch NPCO_CHTP , 1. year of study, winter semester, compulsory

  • Programme NKCP_CHTP Master's

    branch NKCO_CHTP , 1. year of study, winter semester, compulsory

Type of course unit


Guided consultation in combined form of studies

26 hours, obligation not entered

Teacher / Lecturer

Laboratory exercise

39 hours, compulsory

Teacher / Lecturer