Course detail

Biochemistry II

FCH-MCO_BCH2Acad. year: 2021/2022

Metabolic pathways . Bioenergetics and oxidative metabolism. Short-term and long-term control mechanisms - regulation of enzyme activity, gene expression regulation; cell signaling. Carbohydrate metabolism - major metabolic pathways and their control, special pathways. Tricarboxylic acid cycle. Electron transport chain. Oxidative phosphorylation. Lipid metabolism - utilization and storage of energy. Metabolismus of nitrogen compounds I - Amino acid metabolism. Metabolsim of nitrogen compounds II - Porfyrin and heme metabolism, Nucelotide metabolism. Metabolic interrelationships and control. Compartmentation. Membrane transport. Biochemistry of hormones and the hormonal cascade system.

Language of instruction


Number of ECTS credits


Mode of study

Not applicable.

Learning outcomes of the course unit

The course Biochemistry II is focused on detailed study of metabolic pathways and their regulation on molecular as well as cellular level. The knowlege obtained after passing the course can serve as basis for further studies, especially for biotechnology, molecular biology and bioengineering.


Examination - Biochemistry I.


Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Examination - oral; discussion of 3 questions form the whole problematics..

Course curriculum

1. Basic principles of metabolism - overview.
2. Metabolic control mechanisms I - regulation of enzyme activity, cell signaling.
3. Metabolic control mechanisms I I - regulation of gene expression, protein turnover.
4. Carbohydrate metabolism - glycolysis, fermentation.
5. Metabolsim of fructose and galactose. Glycogene metabolism and its control. Hormones.
6. Tricarboxylic ycid cycle. Glyoxalate cycle. Glucoenogenesis.
7. Metabolism of lipids and fatty acids. The ketone bodies.
8. Biological oxidations. Electron transport chain and oxidative phosphorylation. Photosynthesis.
9. Isoprenoid pathway. Cholesterol metabolis.
10. Nitrogen metabolism I - fixation, metabolism of amino acids, the urea cycle.
11. Nitrogen metabolism II - porphyrins, heme metabolism. Nucleotides metabolism.
12. Secondary metabolism - overview.

Work placements

Not applicable.


The aim of the course Biochemistry II is to introduce basic principles of cell metabolism and its regulation. Based on general thermodynamic principles the course of central biochemical pathways including metabolism of saccharides, fatty acids and amino acids is elucidated. Further, basic principles of biological oxidations, cell energy production and photosynthesis are discussed. Next, metabolism of nucleic acids and proteins and processes involved in gene expression are introduced. As a part of course intermediate metabolism and complex regulation of metabolic pathways are presented.

Specification of controlled education, way of implementation and compensation for absences


Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Vodrážka Z.: Biochemie. Academia Praha, Praha 1999. (CS)
Stryer L.: Biochemistry. New York 1988. (CS)
Kodíček M., Valentová O.: Biochemie, VŠCT Praha, 2.vydání (CS)

Recommended reading

Alberts B., Bray D., Johnson A., Lewis J., Raff M., Robertson K., Walter P.: Základy buněčné biologie. Espero Publishing, Ústí n. Labem 2005. (CS)
Voet D., Voet J. G.: Biochemie. Victoria Publishing a.s., Praha 1995. (CS)

Classification of course in study plans

  • Programme NPCP_CHTP Master's

    branch NPCO_CHTP , 1. year of study, winter semester, compulsory

  • Programme NKCP_CHTP Master's

    branch NKCO_CHTP , 1. year of study, winter semester, compulsory

  • Programme NPCP_MA Master's

    branch NPCO_MA , 1. year of study, winter semester, compulsory

Type of course unit



26 hours, optionally

Teacher / Lecturer

Guided consultation in combined form of studies

26 hours, obligation not entered

Teacher / Lecturer